摘要
目的研究白花丹醌对瘦素刺激体外培养人HSC-LX2TGF-β1 mRNA和蛋白表达的影响。方法体外培养HSC-LX2,瘦素(leptin)刺激24h,各药物与细胞共孵育24h后,采用荧光PCR检测各组TGF-β1 mRNA的表达和免疫组织化学方法检测TGF-β1蛋白表达情况。结果与leptin组比较,白花丹醌各剂量组作用24h后,HSC-LX2细胞中TGF-β1 mRNA的水平明显降低,尤以中、高剂量组明显(P<0.01);免疫组化结果显示白花丹醌各剂量组对HSC-LX2细胞TGF-β1蛋白表达有一定的抑制作用,且作用呈剂量依赖性。结论白花丹醌抗肝纤维化作用机制之一是从mRNA和蛋白水平抑制TGF-β1表达,从而抑制HSCECM的合成,发挥抗肝纤维化作用。
Aim To investigate the effect of plumbagozeylanica (plumbago) on the expression of TGF-β1 protein and TGF-β1mRNA in human hepatic stellate cells(HSC-LX2) activated by leptin.Methods HSC-LX2 were cultured in vitro,stimulated by leptin for 24 hours and treated with different concentrations of plumbago for 24 hours,The expressions of TGF-β1mRNA were determined by Real-time quantitative PCR using SYBR Green I,and the expressions of TGF-β1were determined by immunohistochemistry. Results The expressions of TGF-β1mRNA were significantly reduced by the treatment with three concentrations of plumbago than that in the leptin group,especially in middle and high dose group(P0.01); the result of immunohistochemistry showed that expression of TGF-β1 in the plumbago-treatment groups was to some extent lower than that in the leptin group (P0.05),and the inhibitory effect was in a dose-dependent manner.Conclusion The expressions of TGF-β1can be down-regulated by plumbago,resulting in the reduction of the synthesis of ECM and the promotion of the degradation of ECM,which may be one of the possible molecular mechanisms against hepatic fibrosis.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2010年第6期710-713,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30760321)
广西科学研究与技术开发项目资助课题(No桂科攻No0992003-1)
关键词
白花丹醌
瘦素
肝星状细胞
肝纤维化
增殖
转化生长因子-Β1
plumbago
leptin
human hepatic stellate cells
hepatic fibrosis
proliferation
transforming growth factor beta 1