莲心碱对血小板聚集、凝血功能和血栓形成的影响

Effects of liensinine on platelet aggregation and coagulability and thrombotic activity

目的探讨莲心碱对大鼠体内血小板聚集和凝血功能的影响,同时评价其抗血栓作用。方法以二磷酸腺苷(ADP)诱导血小板聚集,采用比浊法观察莲心碱对大鼠体内血小板1、5min聚集率和最大聚集率的影响;通过毛细管法和减尾法分别研究莲心碱对小鼠凝血时间和尾出血时间的影响,同时评价莲心碱对大鼠凝血酶原时间(PT)、活化部分凝血激酶时间(APTT)及凝血酶时间(TT)的影响;采用Chandler法及动静脉旁路模型研究莲心碱对大鼠体外血栓和动静脉旁路血栓形成的影响。结果莲心碱5mg·L-1和10mg·L-1均能明显抑制ADP诱导的大鼠体内血小板1、5min聚集率和最大聚集率;明显延长大鼠PT、APTT和TT;明显延长小鼠凝血时间及尾出血时间;不同程度抑制大鼠体外血栓和动静脉旁路血栓形成,减轻血栓湿重和干重。结论莲心碱可明显对抗血栓形成,并具有对抗血小板聚集和凝血作用。

Aim To investigate the effects of Liensinine on platelet aggregation and coagulation function in rats,as well as the effect on experimental thrombosis.Methods Inhibition rates of platelet aggregation for Liensinine in vivo were determined by the model of platelet aggregation induced by adenosine diphosphate.Coagulation time of mice was measured by capillary vessel method,and bleeding time of mice was measured by tail-cutting method.The effects of Liensinine were also evaluated on prothrombin time（PT）,activated partial thromboplastin time（APTT）and thrombin time（TT）.The model of artery-vein bypass thrombosis and Chandler＇s model were established to observe the effect of Liensinine.Results The result showed that Liensinine 5 and 10 mg·kg-1 had significant effect on inhibition of platelet aggregation and markedly prolonged bleeding time,coagulation time,PT,APTT and TT.Liensinine 5 and 10 mg·kg-1 inhibited the artery-vein bypass and Chandler＇s thrombus in different degree,reduced the thrombus weight significantly （either wet ordry）.Conclusion Liensinine exerts remarkable effect against thrombosis and possesses strong effect against platelet aggregation and coagulation.