茶树花多糖免疫调节与抗肿瘤活性的研究(英文)

Immunomodulatory and Antitumor Activity of Polysaccharide Isolated From Tea Plant Flower

茶树花富含多糖,是茶树生长过程中的产物,然而目前茶树花多糖(tea plant flow polysaccharide,TFP)尚未被充分利用.因此,认为茶树花多糖有着和茶叶中的多糖相似的功能效果.研究目的是通过系列的体内和体外实验系统来评价茶树花多糖的抗肿瘤以及免疫调节的活性.利用S180(sarcoma180)荷瘤小鼠模型,系统地研究了灌胃不同剂量茶树花多糖对荷瘤小鼠的肉瘤抑制率、存活率以及细胞免疫的影响.结果显示,连续灌胃10天茶树花多糖,能显著抑制S180肉瘤的生长,延长荷瘤小鼠的存活时间,增强迟发型超敏作用(delayed-type hypersensitivity,DTH),促进血液白介素-2(IL-2)、γ-干扰素(IFN-γ)的分泌,增强巨噬细胞的吞噬作用,改善T淋巴细胞亚群CD4+数量以及CD4+/CD8+的比值,表明茶树花多糖能增强机体对肿瘤的防御,在一定程度上归功于其对免疫调节作用.

Tea plant flower is a product of the tea plant during its growing. Polysaccharide are abundant in tea flower. However, the components in tea flower are underutilized at present. The study aimed at evaluating the antitumor activity and immunomodulatory effect of tea plant flower polysaccharide （TFP） via various in vivo assay systems. The inhibition effect of TFP on sarcoma 180 tumor （S180）-bearing mice was observed at dosages of 75, 150 and 300 mg/kg by systematically to measure the S180 tumor inhibition rate, mice survival rate and cellular immunity. The result showed that continuous administration of TFP for 10 day continuously was found to inhibit the growth of transplanted S180, prolong the mice survival days, promote the plasma interleukin-2, interferon-γ levels and improve the T-lymphocyte subsets CD4＋ and CD4＋/CD8＋ percentages after treatment with TFP. In addition, TFP was found to increase the delayed-type hypersensitivity response and macrophage phagocytosis significantly. These results strongly suggest that TFP enhances the host defense response to tumor due in part to the immunomodulatory activity.