furin基因P1启动子区-229C/T多态性与肝硬化患者肝细胞功能的关系

Relationship between single nucleotide polymorphism-229C/T in P1 promoter of furin gene and functions of hepatocytes in patients with liver cirrhosis

目的:探讨furin基因启动子活性对肝硬化患者肝细胞功能的影响。方法:收集180例乙型肝炎肝硬化患者的血样和临床资料,采用竞争分化聚合酶链反应技术对furinP1启动子区单核苷酸多态性(SNP-229C/T)进行基因分型,并以其为活性指标分析启动子活性与肝脏血清酶学、胆红素、白蛋白和凝血酶原等水平的关系。结果:全部患者中,等位基因C的频率为75.3%(271/360),等位基因T的频率为24.7%(89/360),基因型CC、CT和TT的频率分别为62.2%(112/180)、26.1%(47/180)和11.7%(21/180)。SNP-229C/T基因分型与主要血清酶学、胆红素、白蛋白和凝血酶原等水平无关,而与碱性磷酸酶和γ-谷氨酰转移酶有关。结论:furin基因启动子的活性对肝细胞主要功能无影响,提示furin作为HBV感染的治疗新靶点具有一定的可行性。

AIM： To study the influences of P1 promoter activity offurin gene on the functions of hepatocytes in patients with liver cirrhosis. METHODS： The patients with liver cirrhosis of 180 cases were recruited. The single nucleotide polymorphism （SNP -229 C/T） in P1 promoter offurin gene was genotyped using competitively differentiated polymerase chain reaction. The relationships between the promoter activity based on genotyping and the serum levels of liver enzymes, total bilirubin, albumin and prothrombin were observed. RESULTS ： The distribution frequencies of allele C and T were 75. 3% （271/360） and 24. 7% （89/360）. Those of genotypes CC, CT and TT were 62. 2% （112/180）, 26. 1% （47/180） and 11.7% （21/180）, respectively. The distribution frequencies of the genotypes were not related to the serum levels of major liver enzymes, albumin, total bilirubin and prothrombin, except for alkaline phosphatase and γ- glutamyl transferase. CONCLUSION ： The activity offurin promoter exerts no effects on the main functions of hepatocytes, suggesting that furin may be a new therapeutic target for HBV infection.