转移相关基因cd44v在胃癌中的表达及其临床意义

The Expression and Clinical Significance of Metastasis related Gene cd44v in Gastric Cancer

为探讨ｃｄ４４ｖｍＲＮＡ拼接和异常表达与胃癌临床病理之间的相关意义，用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）方法检测正常胃粘膜、癌旁组织及癌组织的转移相关基因ｃｄ４４ｖｍＲＮＡ表达。对４０份胃组织标本（２０份胃癌组织，１０份癌旁组织，１０份正常胃粘膜）中ｃｄ４４ｖ变异表达产物进行检测。结果显示：有ｃｄ４４ｖｍＲＮＡ阳性表达者：胃癌组织１６／２０，癌旁组织５／１０，正常粘膜０／１０；有淋巴转移者１５／１６，无淋巴结转移者１／４，表达有差异（Ｐ＜０．０５）；有远处转移者５／５。所有４０份胃癌组织、癌旁组织、正常粘膜都表达标准型ｃｄ４４（ｃｄ４４ｓ），而且两型胃癌中ｃｄ４４ｖ表达有差异（Ｐ＜０．０５），高分化腺癌（肠型）中２／５，低分化癌（弥漫型）中１４／１５。提示ｃｄ４４ｖｍＲＮＡ在胃癌中的异常表达有普遍性，既可能是早期癌变出现的生物学指标，也可能是判断胃癌转移的有用指标。

To study the correlation of the cd44 gene splice and abnormal expression with the clinicopathologic changes, the expression of cd44v mRNA in the normal gastric mucosa, cancer and its adjacent tissues by RT PCR were detected. The results showed that cd44v mRNA was expressed in 16 of 20 gastric cancers, in 5 of 10 the adjacent tissues, but was not expressed in all the 10 normal mucosa specimens; and 1 out of 4 had no lymph node metastasis, all the five cases investigated showed distant metastasis( P <0.05). There were differences in the expression between two types of gastric mucosa( P<0.05 ): 2 out of 5 were well differentiated or intestinal type gastric cancers, 14 out of 15 were poorly differentiated or diffuse type cancers. All the 40 gastric mucosa, gastric cancer and their adjacent tissues presented the standard cd44s mRNA. The conclusion is that the abnormal expression of cd44v is of universal significance in gastric cancer. These might serve as early markers of gastric cancer and its metastasis.