摘要
目的:探讨腹水中生存素(survivin)、血管内皮生长因子(vascular endothelial growth factor,VEGF)在良、恶性腹水鉴别诊断中的价值,以及二者联合检测的临床意义。方法:收集腹水标本62例,分为良性腹水组(22例)和恶性腹水组(40例),采用ELISA法测定survivin和VEGF。结果:恶性腹水组survivin、VEGF水平明显高于良性腹水组(P<0.01)。卵巢癌引起的腹水中survivin、VEGF水平显著高于胃癌组、胰腺癌组、肝癌组(P<0.05)。survivin诊断恶性腹水的敏感性为80.0%,特异性为86.4%,诊断准确率为82.3%。VEGF诊断恶性腹水的敏感性为67.5%,特异性为86.4%,诊断准确率为74.2%。同时检测患者腹水survivin和VEGF,其诊断恶性腹水的敏感性为90.0%,特异性为77.3%,诊断准确率为85.5%。survivin的表达与VEGF的表达密切相关(P<0.01)。结论:腹水中survivin、VEGF测定有助于良、恶性腹水的鉴别诊断;恶性腹水中survivin、VEGF的含量对恶性腹水的组织来源可能有一定的鉴别诊断作用;联合检测恶性腹水中的survivin、VEGF水平可明显提高诊断恶性腹水的敏感性。
Objective: To investigate the values of survivin and vascular endothelial growth factor (VEGF) for differentially diagnosing ascites and the clinical significance of the combined detection of the tumor markers. Methods: A total of 62 ascites samples were collected, with benign ascites in 22 cases and malignant ascites in 40. The levels of survivin and VEGF were determined using ELISA. Results: The survivin and VEGF levels were significantly higher in the malignant ascites than in the benign ascites (P〈0.01). When compared across groups, the survivin and VEGF levels were significantly higher in the malignant ascites of the group with ovarian carcinoma compared to the groups with gastric carcinoma, pancreatic carcinoma or liver cancer (P〈0.05). The sensitivity, specificity and diagnostic accordance rate of survivin in diagnosis of malignant ascites were respectively 80%, 86.4% and 82.3%, those of VEGF were 67.5%, 86.4%, and 74.2%, and those of survivin and VEGF in the combined detection were 90%, 77.3% and 85.5%. The expression of survivin closely correlated to that of VEGF (P〈0.01). Conclusions: The detection of the survivin and VEGF levels in ascites can contribute to distinguish benign ascites from malignant ascites. This, combined with histogenesis detection, can significantly raise the sensitivity in the diagnosis of ascites.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2010年第11期623-625,共3页
Chinese Journal of Clinical Oncology
关键词
腹水
生存素
血管内皮生长因子
鉴别诊断
Ascites
Survivin
Vascular endothelial growth factor (VEGF)
Differential diagnosis