红细胞CR1和CD58数量的变化与胃良恶性溃疡的关系探讨

Relationship of the changes of RBC-CR1 and RBC-CD58 with benign and malignant gastric ulcer

目的探讨良恶性胃溃疡患者红细胞CR1、CD58的表达及其与T细胞亚群变化的关系,为其免疫防治提供理论依据。方法采用流式细胞仪、免疫粘附法和SAP法检测良恶性胃溃疡患者红细胞CR1和CD58的数量、CR1的活性及T细胞亚群的变化。结果 35例恶性胃溃疡组红细胞CR1、CD58、C3bRR和TRR明显下降,显著低于38例良性胃溃疡组和32例正常对照组(P<0.01)。CD3+、CD4+和CD4+/CD8+在恶性胃溃疡组也显著低于良性胃溃疡组和正常对照组(P<0.01)。RBC-ICR和CD8+在恶性胃溃疡组最高,良性胃溃疡组次之,对照组最低(P<0.05)。良恶性胃溃疡组红细胞CR1的数量和活性、CD58的数量与T细胞亚群(CD4+/CD8+)呈正相关(P<0.05)。结论红细胞CR1、CD58与T细胞亚群可能参与了良、恶性胃溃疡的发生与发展。红细胞免疫可能通过CD58调控了包括T细胞亚群在内的某些免疫细胞的活性,从而共同发挥免疫防御作用。

Objective To study the expression of CR1,CD58 and the correlation of them with T-lymphocyte subsets in patients with benign and malignant gastric ulcer and to provide a theoretical basis for immune therapy.Methods Flow cytometry,immune adherence rosette method and SAP assay were used to measure the quantities of RBC-CR1 and RBC-CD58,the activity of RBC-CR1 and the changes of T-lymphocyte subsets in the benign and malignant gastric ulcer group.Results The value of RBC-CR1,RBC-CD58,RBC-C3bRR and RBC-TRR were remarkably lower in the malignant gastric ulcer group than those in the benign gastric ulcer group and control group（P0.01）.The percentage of CD3＋,CD4＋ and the CD4＋/CD8＋ ratio were significantly lower in the malignant gastric ulcer group compared with the benign gastric ulcer group and control subjects（P0.01）.The value of RBC-ICR and CD8＋ were the highest in the malignant gastric ulcer group.They were secondly in the benign gastric ulcer group and the lowest in the control group（P0.05）.The CD4＋/CD8＋ ratio was positively correlated with the quantities of RBC-CR1,RBC-CD58 and the activity of RBC-CR1 in the benign and malignant gastric ulcer group（P0.05）.Conclusion RBC-CR1,RBC-CD58 and T-lymphocyte subsets may be involved in the occurrence and development of the benign and malignant gastric ulcer.Erythrocyte immunity may regulate the activity of immunocytes including T-lymphocyte subsets by CD58.Erythrocyte immunity and T-lymphocyte subsets may have the immune prevention functions in patients with benign and malignant gastric ulcer.