二甲亚砜诱导人支气管上皮细胞炎症因子胸腺基质淋巴细胞生成素的表达

Dimethylsulfoxide-induced expression of thymic stromal lymphopoietin in human bronchial epithelial cells

目的探讨二甲亚砜(DMSO)对人支气管上皮细胞炎症关键分子——胸腺基质淋巴细胞生成素(TSLP)表达的影响。方法用不同浓度DMSO(0.1%、1%、2.5%)孵育人支气管上皮细胞,分为对照组和各浓度组,收集细胞及上清液后,采用实时定量PCR检测细胞TSLPmRNA表达水平,ELISA技术检测细胞培养上清中TSLP浓度。结果 DMSO显著诱导人支气管上皮细胞TSLPmRNA表达(P<0.01),并呈浓度依赖性。分泌型TSLP水平也随之增高(P<0.01)。高浓度DMSO对细胞生长有抑制作用。结论 DMSO可能影响了支气管上皮细胞的免疫调节功能。

Objective To investigate the effect of dimethylsulfoxide on the expression of thymic stromal lymphopoietin （TSLP） in human bronchial epithelial cell （HBE）. Methods 16HBE cells were incubated in the presence of dimethylsulfoxide at different concentrations, and the cell proliferation changes were observed. The expressions of TSLP mRNA and protein in the cells were detected by real-time quantitative PCR and ELISA, respectively. Results Dimethylsulfoxide induced significantly increased TSLP mRNA expression in HBE cells （P0.01） in a concentration-dependent manner. The level of TSLP protein in the supernatant was also increased after dimethylsulfoxide treatment, but high concentration of dimethylsulfoxide resulted in e inhibited cell proliferation. Conclusion Dimethylsulfoxide may affect the immunomodulatory function of HBE cells.