肝细胞癌组织中microRNA表达谱研究

MicroRNA profiling in patients with hepatocellular carcinoma

目的检测人肝癌组织和对应的肝硬化组织microRNA表达的差异谱,为研究microRNA在肝细胞癌变机制中的作用提供实验基础。方法 Trizol法提取25例配对的肝癌和肝硬化组织总RNA,microRNA芯片分析肝癌和肝硬化组织中差异表达microRNA,实时定量PCR验证芯片的结果。结果肝癌与肝硬化组织间差异表达的microRNA共12个,其中3个表达上调,9个表达下调。实时荧光定量PCR结果与芯片一致。结论肝癌和肝硬化组织存在差异表达的microRNA,这些上调和下调表达的microRNA可能参与了肝癌的发生。

Objective To investigate the differential expression of microRNAs between human hepatocellular carcinoma （HCC） and liver cirrhosis （LC）. Methods The total RNA was extracted from 25 pairs of HCC and LC tissues. microRNA microarray was used to analyze the microRNA expression profiles, and validation of the microarray results was carried out by real-time quantitative RT-PCR （qRT-PCR）. Results Three microRNAs exhibited higher expression in the HCC samples than in the LC samples. In comparison with the LC samples, the HCC samples showed down-regulated expressions of 9 microRNAs. qRT-PCR verified that has-miR-122a, has-miR-199a, and has-miR-199b were downregulated in HCC, which was in agreement with the microarray results. Conclusion HCC and LC samples have significantly different microRNA expression profiles. These differentially expressed microRNAs may be involved in the pathogensis of HCC.