Hath1基因抑制结肠癌细胞的增殖

Hath1 gene transfer inhibits the proliferation of colonic cancer cells in vitro

目的研究Hath1基因对结肠癌细胞增殖的影响。方法将重组质粒pcDNA3.1(+)-Hath1转化至HT29结肠癌细胞中,随机挑选3个过表达Hath1的HT29细胞克隆,通过定量实时RT-PCR方法检测Hath1 mRNA、Muc2mRNA的表达;Western blotting检测Hath1、Muc2、cyclinD1和p27表达;通过软琼脂克隆形成实验和裸鼠移植实验,观察Hath1基因对HT29细胞增殖能力的影响。结果 Hath1基因可上调p27和下调cyclinD1的表达,抑制HT29细胞的增殖。结论 Hath1基因可能在结肠癌的发病中扮演抗癌基因的作用。

Objective To study the effect of Hath1 gene transfer on the proliferation of colonic cancer cells in vitro. Methods The recombinant plasmid pcDNA3.1（＋）-Hath1 was transfected into HT29 colonic cancer cells, and 3 positive cell clones were randomly selected to test the levels of Hath1 mRNA, Muc2 mRNA, Hath1, Muc2, cyclin D1 and p27 by quantitative real-time RT-PCR and Western blotting. The proliferation of the transfected HT29 cells was observed by means of colony formation assay and xenograft growth in nude mice. Results Hath1 significantly down-regulated of cyclin D1 and up-regulate of p27 expressions and inhibited the proliferation of HT29 cells. Conclusion Hath1 gene may be an anti-oncogene in colon carcinogenesis.