不同浓度伊马替尼对大鼠C6胶质瘤细胞凋亡和细胞周期的影响

Effect of imatinib at different concentrations on rat C6 glioma cell apoptosis and cell cycle

目的研究伊马替尼对大鼠C6胶质瘤细胞凋亡和细胞周期的影响。方法采用MTT法测定伊马替尼对C6胶质瘤细胞生长曲线的影响。用Hochest/PI染色法和流式细胞仪观察伊马替尼不同浓度(0.156、10和15μmo/L)作用前后,大鼠C6胶质瘤细胞凋亡和细胞周期的变化。结果伊马替尼15μmo/L可显著影响C6胶质瘤细胞的生长曲线。伊马替尼诱导C6细胞凋亡呈时间-剂量依赖性;在72h时间点,10和15μmo/L组G1/G0期细胞比例增加,分别达到(68.53±0.83)%和(70.41±0.62)%(P<0.01);G2期细胞比例分别降至(14.48±0.12)%和(13.84±2.86)%(P<0.01);S期细胞比例分别降至(16.98±0.72)%和(15.78±2.28)%(P<0.01)。结论伊马替尼可诱导大鼠C6胶质瘤细胞凋亡,改变细胞周期的分布。

Objective To investigate the effect of imatinib on rat C6 glioma cell apoptosis and cell cycle. Methods MTT assay was used to determine the OD value of C6 glioma cells following treatment with imatinib at different concentrations （0.156, 10 and 15 μmo/L） for 24, 48 and 72 h. The cell apoptosis was assayed by Hochest/PI staining and the cell cycle changes were analyzed by flow cytometry. Results Imatinib treatment resulted in increased number of apoptotic cells in a timeand dose-dependent manner. A 72-h treatment of the cells with imatinib at 10 and 15 μmo/L caused increased cell percentage in G0/G1 phase to （68.53±0.83）% and （70.41±0.62）%, （P0.01）, decreased the percentage of G2phase cells to （14.48±0.12）% and （13.84±2.86）% （P0.01）, and decreased the percentage of S phase cells to （16.98±0.72）% and （15.78±2.28）%, respectively （P0.01）. Conclusion Imatinib can induce apoptosis and affect the distribution of the cell cycle of C6 cells in vitro.