舒芬太尼预处理对大鼠急性胃粘膜病变的保护作用及与酸敏感离子通道的关系

Protective effects of sufentanil pretreatment against acute gastric mucosal lesion in rats and its relationship with acid-sensing ion channels

目的观察舒芬太尼预处理对急性胃粘膜病变大鼠胃粘膜的保护作用及其对胸段脊髓背根神经节(DRG)神经元中酸敏感离子通道(ASIC3)表达的影响,探讨其保护机制与ASIC3的关系。方法选取雄性Wistar大鼠,分3组:正常组、浸水束缚应激模型组和舒芬太尼预处理组。采用浸水束缚应激(WIRS)法复制急性胃粘膜病变模型,于应激6h后观察大体胃粘膜损伤程度及溃疡指数,并采用免疫荧光法观察ASIC3在DRG神经元中的表达,采用RT-PCR法进行实时定量研究。结果与正常组比较,WIRS明显损伤胃粘膜,升高溃疡指数,且胸段DRG神经元上ASIC3广泛表达;舒芬太尼预处理可明显减轻WIRS大鼠的胃粘膜损伤程度,降低溃疡指数,并使ASIC3mRNA在胸段DRG神经元的表达量明显减少。结论 ASIC3参与了急性胃粘膜病变的发生机制,舒芬太尼预处理对急性胃粘膜病变具有保护作用,其机制与抑制ASIC3的表达有关。

Objective To investigate the effects of sufentanil pretreatment on acute gastric mucosal lesion and its impact on the expression of acid-sensing ion channel 3 （ASIC3） in thoracic dorsal root ganglia （DRG） neurons in rats with water immersion-restraint stress （WIRS）. Methods Twenty-four Wistar rats were randomly assigned into 3 groups, namely the normal control group （n=6）, WIRS group （n=12） and sufentanil pretreatment group （n=6）. Gastric mucosal lesion was induced by WIRS, and after 6 h of WIRS, the gastric tissues were excised and observed under microscope, with the ulcer index （UI） calculated. The expression of ASIC3 in the DRG neurons was detected by immunofluorescence assay, and the ASIC3 mRNA expression by quantitative real-time RT-PCR. Results Compared with the normal control group, the rats in the WIRS group showed obvious gastric injury with increased UI and extensive expression of ASIC3 in the DRG neurons. Sufentanil pretreatment of the rats subjected to WIRS significantly alleviated the gastric mucosal injury, lowered the UI, and reduced ASIC3 mRNA expression in thoracic DRG neurons. Conclusion ASIC3 is involved in the development of acute gastric mucosal lesion, and sufentanil pretreatment offers protection of gastric mucosa by inhibiting the expression of ASIC3.