慢性髓系白血病不同病期基因表达谱研究

Differential Expression Genes in Various Phases of Chronic Myeloid Leukemia by cDNA Microarray

本研究旨在探讨慢性髓系白血病(chronic myeloid leukemia,CML)急变的分子机制。采用cDNA微重排方法对4例CML急变期、4例CML慢性期患者进行基因差异表达分析。结果显示,共筛选出至少在3张芯片有差异表达的基因74条,其中下调52条,上调22条;差异表达基因包括:细胞骨架/运动相关基因、信号传导相关基因、转录因子相关基因、免疫相关基因、代谢相关基因、细胞周期相关基因、原癌和抑癌基因、细胞受体相关基因、蛋白质翻译合成相关基因及功能未知的基因等。结论:急变是多基因异常相互作用的结果,其中功能异常的信号转导、细胞周期调控、细胞分化及免疫的相关基因可能是导致CML急变的关键基因。

The aim of this study was to explore the mechanism underlying the blast crisis of chronic myeloid leukemia. Analysis of gene expression profiles of chronic myeloid leukemia patients in chronic phase and blast crisis were analyzed by using cDNA microarray representing 4096 genes for finding the differential expression genes. The results indicated that 74 differential expression genes were identified in at least 3 gene chips in blast crisis compared with chronic phase, among them 52 genes were down-regulated and 22 genes were up-regulated in blast crisis. The differential expression genes were involved in these genes including genes related to cell structure/mobility, signal transduction, transcription factor, related immunity, metabolism, cell cycle, oncogene/anti-oncogene, cell receptor, protein translation/synthesis and some unknown functions. It is concluded that the blast crisis of CML is resulted from abnormality and interaction of multigenes, among them functional abnormal genes related to signal transduction, cell cycle, cell differentiation and immunity may be the critical genes for chronic myeloid leukemia blast crisis.