急性淋巴细胞白血病患者免疫功能评价初步研究

Preliminary Evaluation of Immunological Function in Patients with Acute Lymphocytic Leukemia

本研究旨在采用蛋白质芯片及酶联免疫吸附试验(ELISA)方法对急性淋巴细胞白血病(ALL)患者的IL-2、IL-4、IL-12、IL-13、TNF-α、IFN-γ6种免疫相关细胞因子进行测定,以评价患者的免疫功能。制作人细胞因子蛋白质芯片,通过芯片及ELISA法对收集的患者及正常人外周血的血清(其中患者28例,正常对照25例)进行测定。结果表明,ALL患者IL-4、IL-13、IL-12、IFN-γ及TNF-α表达水平均低于正常组,但ELISA检测显示,TNF-α表达在ALL和正常对照组中均为阴性。结论:ALL患者的细胞免疫与体液免疫均受到抑制,芯片比ELISA方法敏感。

This study was aimed to investigate 6 kinds of human cytokines （IL-2, IL-4, IL-12, IL-13, IFN-γ/and TNF- α） in patients with acute lymphocytic leukemia （ALL）, so as to find their relationship with the disease. The pure monoclonal antibodies were spotted on the prepared NC membrane glass slides under certain conditions to make human cytokine protein microarray. The serum samples were collected from peripheral blood of 28 patients and 25 normal controls, the serum concentration of cytokines was determined by using the protein microarray and ELISA. The results showed that the expression levels of IL-4, IL-12, IL-13, IFN- γand TNF- α in ALL patients were lower than those in the normal controls, but ELISA indicated that the expression of TNF -α in ALL patients and normal controls was negative. In conclusion, both cellular and humoral immunity are all inhibited in patients with ALL. Microarray is more sensitive than ELISA.