CD4^+ CD25^+调节性T细胞和细胞因子与异基因造血干细胞移植后aGVHD的相关性研究

Relationship Between CD4^+ CD25^+ Regulatory T cells,IL-2,TGF-β and Acute Graft-Versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation

本研究旨在研究异基因造血干细胞移植(allo-HSCT)后患者外周血CD4+CD25+调节性T细胞(Treg)、细胞因子IL-2、TGF-β水平与受者急性移植物抗宿主病(aGVHD)的相关性。采用流式细胞术检测13例allo-HSCT患者术后外周血Treg细胞在CD4+T细胞中的百分比;用ELISA法检测其血清IL-2和TGF-β水平。结果表明:13例患者均获造血重建,non-aGVHD4例,Ⅰ-Ⅱ度aGVHD5例,Ⅲ-Ⅳ度aGVHD4例。non-aGVHD组Treg在CD4+T细胞中所占的百分比高于aGVHD组,差异有显著性(p<0.05);non-aGVHD组血清IL-2水平低于aGVHD组,差异有显著性(p<0.05);non-aGVHD组血清TGF-β水平高于aGVHD组,差异有显著性(p<0.05)。结论:外周血Treg、IL-2、TGF-β水平与allo-HSCT后aGVHD的发生及严重程度有密切关系,因此可作为早期判断和监测aGVHD的指标。

This study was aimed to investigate the relationship between CD4 ^＋ CD25 ^＋ regulatory T cells, IL-2, TGF- β and acute graft-versus host disease （aGVHD） after ailogeneic hematopoietic stem cell transplantation （allo-HSCT）. The percentage of peripherial blood CD4 ^＋ CD25 ^＋ Treg cells in CD4 ^＋ T cells of 13 patients with hematological malignancies after allo-HSCT were detected by flow cytometry; serum levels IL-2 and TGF-β in these patients were measured by ELISA. The results indicated that all the patients achieved engraftment. 5 patients developed aGVHD of grade Ⅰ - Ⅱ , 4 patients developed aGVHD of grade Ⅲ - Ⅳ. The percentage of pefiphefial blood CD4 ^＋ CD25 ^＋ Treg cells out of CD4 ^＋ T cells in patients without aGVHD was higher than that in patients with aGVHD （p 〈 0.05 ） ; the serum level of IL-2 in patients without aGVHD was lower than that of patients with aGVHD（p 〈 0.05 ） ; the serum level of TGF-β in patients without aGVHD was higher than that of patients with aGVHD（p 〈 0.05 ）. It is concluded that CD4 ＋ CD25 ^＋ Treg cell level and the serum level of IL-2 and TGF-β all are related to incidence and severity of aGVHD. These factors may be used as indicators for early evaluating and monitoring aGVHD after allo-HSCT.