碱化血浆紫外线蓝光照射树脂吸附清除胆红素

Clearance of bilirubin by ultraviolet-blue ray irradiation and resin adsorption on alkalified plasma

目的探讨用紫外线蓝光混合光照射碱化血浆再用树脂吸附对胆红素影响,建立净化人工肝分离血浆新方法。方法 (一)将人工肝分离出的血浆碱化成pH10(血浆:0.2mol/LNa2CO3=1∶1,V/V),用紫外线蓝光照射(10～14)min,再用D4006(交联PS塑料)非极性吸附树脂流动吸附3min,检测胆红素和蛋白浓度。(二)将人工肝分离出的血浆碱化成pH10(血浆:2mol/LNa2CO3=10∶1,V/V),用紫外线蓝光照射15min,再用D4006树脂流动吸附5min,检测胆红素和蛋白浓度。结果在方法一,碱化血浆照射组的胆红素(TBil、DBil、IBil)清除率分别为86%、84%、87.5%,Alb回收率99%;碱化血浆照射吸附组的TBil、DBil、IBil清除率均为98%,Alb回收率90%。在方法二,碱化血浆照射组的TBil、DBil、IBil清除率分别为85%、84%、86%,Alb回收率99%;碱化血浆照射吸附组的TBil,DBil,IBil清除率分别为90%、87%、91%,Alb回收率90%。结论紫外线蓝光照射碱化血浆可明显降低胆红素浓度,照射后再用D4006树脂吸附可进一步降低胆红素浓度,且Alb回收率达90%～99%。故本法有望用于人工肝血液净化。

Objective To study the effect of ultraviolet-blue ray and resin adsorption on bilirubin clearance,and develop a new method to purify plasma from artificial liver treatment. Methods The patients were classified in two groups. In Group A patients the plasma from artificial liver treatment was alkalified to pH 10 （plasma： 0.2mol/L Na2CO3=1∶1,V/V）,irradiated by the ultraviolet-blue ray for 10-14 min and then filtrated by a non-polar adsorbent resin for 3 min （D4006,crosslinked polysyrene）. In Group B,The plasma was alkalified to pH 10 （plasma： 2mol/L Na2CO3=1∶1,V/V）,irradiated by the ultraviolet-blue ray for 15 min and then filtrated by the D4006 resin for 5 minute to decrease the level of bilirubin.Results In group A patients,the clearance rates of total,direct and indirect bilirubin （TBil,DBil,IBil） and the recovery rate of ALB were 86%,84%,87.5% and 99% by irradiation of alkalified plasma,and 85%,84%,86% and 98% by irradiation then adsorption of alkalified plasma. In group B,the clearance rates of total,direct and indirect bilirubin （TBil,DBil,IBil）,and the recovery rate of ALB were 85%,84%,86% and 99% by irradiation of alkalified plasma and 90%,87%,91% and 90% by irradiation then adsorption of alkalified plasma. Conclusion The levels of bilirubin decreased significantly by the ultraviolet-blue ray irradiaton on the alkalified plasma,and the level also decreased further by the irradiation then adsorption by the D4006 resin. This new method may be useful to purify plasma from artificial liver treatment.plasma exchange; bilirubin; resins,synthetic; adsorption; ultraviolet therapy; liver,artificial