5个芍药品种愈伤组织诱导及分化研究

Induction and regeneration of callus tissues in five peony cultivars

芍药组织培养是解决其快速繁殖的有效方法,更为其新品种的繁育提供有效途径。本试验分别从5个芍药品种的不同部位取材,研究了影响芍药愈伤组织诱导和再分化的主要因素。结果表明:2,4-D、NAA与TDZ诱导愈伤组织效果较好,KT、IAA和IBA不利于愈伤组织诱导和不定芽分化;在愈伤组织再分化中,NAA效果优于2,4-D,TDZ优于6-BA;在所选外植体中只有胚轴产生的愈伤组织分化出不定芽,平均分化率为7.95%。

Tissue culture of peony provides an effective approach of improving rapid propagation,especially for new cultivars.Five cultivars were selected to study the induction and regeneration of peony callus.Results show that the addition of 2,4-D,NAA and TDZ was effective in the induction of callus,while KT,IAA and IBA had negative effects in the induction of callus and differentiation of adventitious buds.In terms of regeneration of callus,NAA had a better effect than 2,4-D and TDZ was better than 6-BA.Only callus from the hypocotyl succeeded in producing adventitious buds,with an average regeneration rate of 7.95%.