白藜芦醇对β淀粉样蛋白诱导星形胶质细胞氧化损伤的保护作用

Resveratrol Attenuates β-Amyloid-Induced Oxidative Stress in Astrocytes

目的研究白藜芦醇对β淀粉样蛋白诱导星形胶质细胞氧化损伤的保护作用,从而探索其对中枢神经保护作用的可能机制。方法采用不同浓度的白藜芦醇预处理星形胶质细胞12h,继之用25μmol/L浓度的β淀粉样蛋白进行损伤,然后检测细胞存活率、乳酸脱氢酶(LDH)以及采用DHE探针测定荧光强度间接反映胞内活性氧(ROS)含量,以评价白藜芦醇对细胞氧化损伤的保护作用。结果受β淀粉样损伤的细胞存活率明显下降(P<0.05),而用10、100μmol/L白藜芦醇预处理则能提高该细胞的存活率(P<0.05),但1μmol/L白藜芦醇预处理的细胞存活率并无明显变化;荧光检测显示,蛋白损伤使得荧光强度增高(P<0.05),1μmol/L白藜芦醇处理组荧光强度大于阳性对照组,其余各组荧光强度均小于阳性对照组(P<0.05);蛋白损伤可导致细胞LDH漏出量增加(P<0.05),1μmol/L白藜芦醇使得LDH漏出增加(P<0.05),而10、100μmol/L白藜芦醇处理组,细胞LDH漏出量明显减少(P<0.05)。结论星形胶质细胞受到β淀粉样蛋白损伤后,白藜芦醇能减少LDH的漏出及有效降低胞内ROS水平,从而显示对星形胶质细胞具有保护作用。

Objective To study the effect of resveratrol on β-amyloid-induced oxidative injury in cultured astrocytes and the protective effects of resveratrol on the central nervous system. Methods Astrocytes were treated with various concentrations of resveratrol for 12 h and then exposed to Aβ. Cell viability was detected by CCK-8 assay. ROS was measured using dihydroethidium （DHE） and the fluorescence was measured by multilabel counter. The level of lactate dehydrogenase （LDH） leakage was examined after injury. Results 10,100 μmol / L resveratrol pretreatment was found to significantly increase the cell viability. DHE intensity was decreased when the cells were pretreated with 10,100 μmol / L of resveratrol,whereas 1 μmol / L of resveratrol increased the DHE intensity. LDH leakage was significantly increased after Aβ treatment of the astrocytes,and the LDH leakage was inhibited after resveratrol （10,100 μmol / L） pretreatment. Conclusion Resveratrol could increase the cell viability and inhibit the release of LDH leakage,and β-amyloid-induced oxidative injury can be decreased with resveratrol pretreatment.