猴胎脑组织移植的实验研究

Experimental study of embryonic brain tissue transplantation in monkey

目的为研究胚胎脑组织移植后的组织生长情况。方法对１０只猕猴进行了胎脑组织移植。实验按受体脑损伤的时间分为立即移植组和延迟移植组，移植物为胎脑组织悬液，直接移植至创面。结果术后受体的行为及体温均正常；组织学检查发现，移植后１个月可见受体脑内有部分移植的神经元存活，３个月后发现神经元退行性变，部分神经元消失，６个月后移植部位仅见胶质瘢痕和吞噬细胞浸润，显示排斥反应，其中１只术后４个月仍可见存活的神经元，移植物与受体脑界面有相连的毛细血管，并有突触连接。结论脑组织异体移植仍有严重的排斥反应，立即移植与延迟移植的移植物存活无明显差异。

Objective To study the tissue growth of embryonic brain grafts in monkey.Methods Embryonic brain tissue transplantation was performed on 10 monkeys who were divided into immediate transplanted group and delayed transplanted group. The grafts was the embryonic tissue homogenate and transplanted into the wound.Results The behaviors and body temperature in the monkeys after transplantation were normal. Histologically, it was found that part neurons was survival in the host brain one month after transplantation. Three months later, retrograde degeneration of the neurons was found and part neurons vanished. Six months after transplantation, only neuron degeneration scar tissue colloid and the phagocyte infiltration were seen in the transplant area. However, a monkey was found to have the neuron survival and the connection of neurofibrils between the grafts and host cerebral cortex.Conclusion Allotransplantation might have severe rejection. The survival of the transplants in immediate transplantation and delayed transplantation had no significant difference.