黄芪注射液对大鼠脑出血后神经的保护作用

Neural protective action of astragalus injection in intracerebral hemorrhage rats

目的探讨黄芪对大鼠脑出血灶周围凋亡神经元的保护作用。方法用Ⅶ型胶原酶脑内注入法制作大鼠脑出血模型,对各组大鼠进行神经功能评分,用免疫组织化学法检测Bcl-2和Bax蛋白表达,透射电镜观察大鼠脑出血灶周围神经元的超微结构,并对神经突触进行定量分析。结果与模型组比较,各治疗组Bcl-2的阳性表达均明显升高(P<0.01);各治疗组Bax的阳性表达均明显降低(P<0.01);Bcl-2/Bax蛋白比值增高。与术后0h、6h治疗组相比,术后24h治疗组Bcl-2和Bax的阳性表达有显著性差异(P<0.05)。术后0h治疗组与术后6h治疗组相比,Bcl-2阳性表达无显著性差异(P>0.05)。神经元超微结构观察,正常组神经元超微结构正常;模型组神经元超微结构严重破坏;各黄芪治疗组神经元超微结构与模型组相比明显好转。其中出血后0h、6h治疗组的神经元超微结构好于出血后24h治疗组。神经突触定量分析显示,与模型组相比,各治疗组神经突触的数量明显增多(P<0.01);突触间隙明显变窄(P<0.01);突触后致密区明显增厚(P<0.01)。术后24h治疗组与术后0h、6h治疗组相比,各定量参数均有显著性差异(P<0.05)。结论黄芪能保护大鼠脑出血灶周围的神经元,增强神经突触功能,减少神经元凋亡,并且早期用药效果较好。

Objective To study the protective effect of astragalus injection on neurons that surround intracerebral hemorrhage（ICH）in rats. Method ICH model was established by injecting collagenase VII into the brain of rat. These rats in every group were graded according to the neurological function after analepsia. Immunohistochemical staining technique was used to demonstrate expressions of Bcl-2 and Bax protein. The ultrastructure of neurons that surround iutracerebral hemorrhage in rats was observed by transmission electron microscope. Results The positive expression of Bcl-2 in treatment group was higher than that in model group （P 〈 0.01 ）; However the positive expression of Bax in treatment group degraded prominently P 〈 0. 01. The protein ratio of Bcl-2/Bax increased. Comparing with 0 hour,6 hours treatment group after surgery, the positive expression of Bcl-2 and Bax in 24 hours treatment group after surgery had prominently difference（P 〈 0.05）. The positive expression of Bcl-2 in 0 hour treatment group and 6 hours treatment group had no prominently difference（P 〉 0. 05 ）. The uhrastructure of neuron in normal group was common, however, it was destroyed seriously in model group. The ultrastructure of neuron in astragalus treatment group improved obviously compared with model group. The uhrastructure of neuron in 0 hour,6 hours treatment group after ICH was better than in 24 hours treatment group. Quantitation analysis of nerve synapse displayed that the number of nerve synapse increased prominently in treatment group than in model group（ P 〈 0.01 ） ;Meanwhile, the synaptic cleft became narrow conspicuously （P 〈 0.01 ） ; The compact area behind synapse thickened obviously（ P 〈 0. 01 ）. The visual parameters of nerve synaptosome in 24 hours treatment group after surgery were different compared with 0 hour,6 hours treatment group. Conclusion Astragalus can protect neuron around hemorrhagic focus in rat brain and reduce neuron apoptosis. Astragalus can also improve the function of synapse and the effect is better if it is used early.