大鼠肾标本连续切片的三维重建及形态学测量

Three-dimensional reconstruction and morphometry method of rat kidney based on serial sections

目的综合运用目前较成熟的技术,建立一个易施行的、可对大组织标本连续切片整体图像进行三维重建及形态学测量的方法。方法对正常大鼠肾的石蜡包埋标本进行全序列连续切片,HE染色后对切片图像进行数字化采集,经过拼接、配准步骤,建立切片整体图像的数字化数据集,对其内部的血管系统及肾盂进行分割及三维重建,并对重建结果进行形态学测量。结果切片的整体图像清晰,数字化图像数据集配准准确,三维重建结果再现了各种结构的立体特征及其在原组织块内的位置,在三维空间内,可进行无阻挡的自由观察,并且测量出了脉管系统的多项三维形态学指标,包括组织体积构成百分比、动脉长度及分支角度等。结论采用这种方法取得了较为满意的重建结果 ,可为其他组织连续切片的三维重建研究提供借鉴。

Objective To explore an easy-to-perform three-dimensional reconstruction method based on serial section images of large tissue by combination of several new techniques. Methods After the processes of HE stain, digitalization, assembling and alignment, the digitalized image database of long serial sections from a normal rat renal paraffin-embedded tissue was made, in which the vascular system and pelvis were segmented and reconstructed, the morphology figures of constructed result were also measured. Results The image database composed of clear whole images and correct registration reappeared structural pattern of the tissue, and the results displayed the complicated positional relationship of renal structures and could be observed freely, some three dimensional figures including percentage components of kidney, length and angel of arteries were obtained. Conclusion We reported a three-dimensional reconstruction method from serial sections with accepting results. It can also be used in other cases needing stereoscopic information or intuitive observation.