神经细胞尼氏体和神经纤维髓鞘的双重组合染色方法

A noval double-compound staining method showing Nissl bodies and myelin sheath in neuron

目的获得较好显示神经元尼氏体和髓鞘微细结构的方法。方法选用煌焦油紫、甲苯胺蓝、硫堇和橙黄G、磷钨酸双重组合染色法(BTT-OP法),对8只新西兰大白兔脊髓和脊神经节进行染色。结果兔脊髓和脊神经节的神经元尼氏体呈蓝色,细胞核呈黄色,轴索呈蓝色,髓鞘呈浅黄色,神经膜和结缔组织纤维呈蓝色,背景呈淡黄色。结论 BTT-OP法所建立的双重组合染色法,使结构对比清晰、色彩鲜艳、在光镜下能清晰显示神经元的结构特征。

Objective To develope a method for staining Nissl bodies and myelin sheath in spinal cord tissues and the spinal ganglion from 8 New Zealand white rabbits. Methods The double-compound staining dyes composed of brilliant cresyl violet, toluidine blue,thionin,orange G and phosphotungstic acid （BTT-OP method） were investigated. Results The Nissl body,axons, nerve membrane and connective tissue were stained blue, the nerve cell nuclei were stained yellow, the myelin sheath and the background showed slight yellow. Conclusion Double-compound staining method （BTT-OP method ）possesses more vivid color and more striking contrast than the original method, it can demonstrate clearly the structures of neuron under the light microscope.