胸腺内胰岛移植的实验研究

Experimental study on intrathymic islet transplantation

目的观察胸腺内和肾包膜下胰岛移植对移植物存活时间的影响。方法以Ｃ５７ＢＬ／６小鼠为受体，ＢＡＬＢ／ｃ小鼠为供体，肾包膜下胰岛移植分为单纯移植和移植的同时加腹腔内一次性注射兔抗小鼠胸腺细胞血清（ＡＴＳ）两组，胸腺内移植组亦分为单纯移植和移植加用ＡＴＳ两组。结果单纯胸腺内移植组其移植物的存活期为（１９．５±１０．１）天，长于单纯肾包膜下移植组（１４．０±２．１）天；移植的同时加用ＡＴＳ，则肾包膜下移植组移植物的存活期为（４３．０±１１．７）天，胸腺内移植组可达（９３．８±２５．５）天以上，其中６只（６／８）胸腺内移植物获长期存活，并且诱导了受体对供体的特异无反应性。结论胸腺可能为胰岛移植的理想部位，而且在诱导免疫耐受中具有重要作用。

Objective To observe the effects of intrathymic islet transplantation or renal subcapsule islet transplantation on the graft survival.Methods C57BL/6 mice were used as recipients and BALB/c as donors. The recipients receiving intrathymic or renal subcapsule islet transplantation were divided into simple transplantation groups and the group intraperitoneally receiving rabbit antithymocyte serum(ATS) at the time of islet transplantation. Results The median survival time (MST) of simple intrathymic allografts were 19.5 days, longer than that of allografts implanted beneath the renal capsule. Administration of ATS at the time of islet transplantation delayed rejection of islets transplanted to the renal subcapsule (MST, 43.0 days), and intrathymic allografts were superior(MST, 93.8 days). Six of 8 intrathymic grafts with administration of ATS survived permanently, especially a state of donor specific unresponsiveness was induced. Conclusion The thymus may be an immunoprivileged site for islet transplantation and may play an important role during the induction of immune tolerance.