生脉散不同组分配伍对热损伤大鼠肝脏糖皮质激素受体的作用

Effects of Different Assemblages with Components of Shengmai Powder on Glucocorticoid Receptor in Liver of Thermal Injured Rat

目的研究生脉散不同组分配伍对热损伤大鼠肝脏糖皮质激素受体(glucocorticoid receptor,GR)的影响,探讨其调节热损伤大鼠肝脏GR较佳的组分配伍比例。方法采用正交设计的方法 ,每味药的剂量分为3个水平:即不用药(均为0)、低剂量(人参总皂苷为7.1mg;麦冬水提物为17.2mg;五味子水提物为9.6mg)、高剂量(人参总皂苷为14.2mg;麦冬水提物为34.4mg;五味子水提物为19.2mg),正交表L9(34)分别赋值并进行分组,通过直接分析和方差分析,确定调节热损伤大鼠肝脏GR较佳的组分配伍比例。结果在不同组分配伍的生脉散作用后,大鼠肝胞液GR的最大结合容量Bmax(fmol/mgpro)分别为:组1为161±26,组2为271±40,组3为166±66,组4为222±45,组5为192±26,组6为194±23,组7为166±15,组8为165±47,组9为211±79。当人参总皂苷、麦冬水提物、五味子水提物的给药剂量为7.1、17.2及19.2mg时,大鼠肝脏GR水平较高。结论生脉散复方不同组分配伍对调节热损伤大鼠肝脏GR水平最佳的配伍比例为人参∶麦冬∶五味子=3∶3∶4。

Objective To study the effects of different assemblages formed by components of Shengmai Powder （SMP） on glucocorticoid receptor （GR） in liver of thermal injured rat to find the optimal ratio of assembling for GR regulation. Methods With a orthogonal design adopted,the dosage of each component of SMP,including the total saponins of Ginseng （G）,the water extract of lilyturf root （L）,and the water extract of schisandra fruit （S）,was ranked in three levels,namely,no participating,low dosage （G 7.1 mg,L 17.2 mg,S 9.6 mg）,high dosage （G 14.2 mg,L 34.4 mg,S 19.2 mg）. The components were assigned by L9（34） orthogonal table and grouped,the best assembling ratio was determined through direct and variance analysis. Results After being acted by the different assemblages,the maximum binding volume of GR in rat＇s liver cell suspension was 161±26 fmol/mg protein in group 1,271±40 fmol/mg protein in group 2,166±66 fmol/mg protein in group 3,222±45 fmol/mg protein in group 4,192±26 fmol/mg protein in group 5,194±23 fmol/mg protein in group 6,166±15 fmol/mg protein in group 7,165±47 fmol/mg protein in group 8 and 211±79 fmol/mg protein in group 9. The optimal GR level appeared during the dosage of G,L and S was 7.1 mg,17.2 mg and 19.2 mg,respectively. Conclusion The best assembling ratio of SMP for regulating GR in the liver of thermal injured rat was G：L：S=3：3：4.