大黄鱼♀与黄姑鱼♂杂交F_1家系初孵仔鱼的AFLP分析

Amplified fragment length polymorphism analysis on newly hatched hybrid fries between Pseudosciaena crocea ♀ and Nibea albiflora ♂

为了评估大黄鱼♀与黄姑鱼♂杂交F1在良种培育与遗传学研究中的应用潜力,利用AFLP标记研究了双亲基因在2个杂交F1家系(HF1和HF2)初孵仔鱼中的传递和分离方式。8对AFLP选择性扩增引物在两对亲本中分别检出478和446个片段。在HF1中,检出片段包括215条母本特异条带(FSB)、165条父本特异条带(MSB)和98条双亲共有条带(MuB),其中,121条(56.3%)FSB、115条(69.7%)MSB和93条(94.9%)MuB传递给了全部后代,其余片段在后代中发生分离。FSB和MSB分离位点的平均显性表型频率之间没有显著性差异。AFLP标记在HF2的传递与分离和HF1相似,只是分离位点的比例较HF1低,而且检出了2个非亲位点。此外,在HF1和HF2中都出现了较高比例的偏离孟德尔定律的分离位点。这些结果表明,大黄鱼♀×黄姑鱼♂杂交F1初孵仔鱼中同时含有来自双亲的基因;虽然计算结果显示杂交F1在遗传上略偏向于母本,但是父源基因和母源基因没有明显的选择性丢失;母本特异位点多态比例与大黄鱼种内杂交F1相当。研究结果为大黄鱼♀×黄姑鱼♂杂交F1的开发与管理提供了科学依据。

In order to assess the potential applications of hybrid F1 between Pseudosciaena crocea ♀ and Nibea albiflora ♂ in the genetic breeding and the basic research, the inheritance and segregation patterns of AFLP markers in the offspring of two hybrid families（ HF1 and HF2 ）were investigated. Totally ,478 and 446 fragments were detected in two pairs of parents by using 8 selective primer combinations, respectively. In the parents of HF1 ,the amplified fragments included 215 female parent-specific bands（ FSB ）, 165 male parent- specific bands （ MSB ） and 98 mutual bands （ MuB ）. All of these fragments were transmitted to the hybrid offspring. Among these fragments, 121 （56.3%） FSB, 115 （69. 7% ） MSB and 93 （94.9%） MuB were transmitted to all the offspring, while the other loci were segregated in the offspring. The level of polymorphism of the FSB was comparable to that in the intraspecific hybrid F1. There was no significant difference between the average frequency of the segregated FSB and that of the segregated MSB. The inheritance and segregation patterns of AFLP markers in HF2 were similar to those in HF1, except that the proportion of segregated loci in HF2 was lower than that in HF1 and 2 non-parental bands were detected in HF2. In addition, a relative high proportion of loci showing distorted segregation was found in both HF1 and HF2. These results suggested that the newly hatched hybrid fries between P. crocea ♀ and N. albiflora ♂ contained the genome of both parents, and the phenomenon of uniparental gene elimination was not obvious. The findings would serve as the basis for further development and management of the hybrids.