不同蛋白源日粮对肉仔鸡小肠发育及相关酶分泌影响研究

Effect of Different Protein Sources on Small Intestine Development and Related Enzyme Secretion

为探讨游离氨基酸、寡肽和完整蛋白对肉鸡消化道发育、消化酶分泌影响,选择450只健康1日龄AA肉仔鸡,随机分为6组,每组5个重复,每个重复15只鸡,公母各半。分别饲喂6种纯合日粮,其日粮蛋白分别为游离氨基酸、不同比例寡肽氨基酸混合物(25∶75、50∶50、75∶25、100∶0)和完整酪蛋白,分别在7、14、21日龄测定小肠各段绒毛长度、隐窝深度,食糜中胃蛋白酶、胰蛋白酶、糜蛋白酶活性。结果表明:①寡肽对肠绒毛生长有明显促进作用,可以提高绒毛长度,降低隐窝深度,增加吸收面积;②寡肽对蛋白酶分泌有促进趋势,但主要是影响糜蛋白酶活性,对胰蛋白酶、胃蛋白酶活性影响不明显。

To discuss the effect of different protein sources on small intestine development and related enzyme secretion. 450 one-day-age AA broilers were selected and divided into 6 groups with 5 replications and fed with six kinds of dlet,contalning free amino acid,oligopeptide and amino acid mixtures in different ratios （25：75,50：50,75：25,100：0） and the integrity of casein respectively. The villous height and crypt depth was measured at 7,14,21 daysand villous height/crypt depth was calculated. The activity of pepsin,trypsin and chymotrypsin was also tested. The results showed： （1）Oligopeptide increased villous height and decreased crypt depth; （2）Oligopeptide improved protease secretion,especially chymotrypsin,but had no significant effect on trypsin or pepsin.