X射线诱发大鼠淋巴细胞TCR基因突变的剂量-效应关系

The Dose-response Relationship of T-cell Receptor Gene Mutation of Rat Peripheral Blood Lymphocytes Induced by X-ray.

目的探讨X射线诱发大鼠外周血淋巴细胞TCR基因突变的剂量-效应关系。方法以不同剂量（0~3Gy）的X射线照射新鲜分离的大鼠外周血淋巴细胞,PHA、ConA、IL-2协同刺激培养7d,流式细胞仪检测TCR突变频率。结果大鼠淋巴细胞体外培养7d后,TCR突变频率随照射剂量的增加而增加,二者存在良好的剂量-效应关系,最佳拟合曲线为二次多项式模式,回归方程为：TCRMF=1.615＋9.979D＋1.712D2（F=146.781,P〈0.01,R2adj=0.864）。结论本研究结果显示：X射线诱发大鼠外周血淋巴细胞TCR基因突变频率与照射剂量间存在良好的剂量-效应关系。

Objective To investigate the does-response relationship of T-cell receptor from rat peripheral blood lymphocyte induced by X-ray.Methods Freshly isolated peripheral lymphocytes from rat were irradiated with X-ray ranged from 0 Gy to 3 Gy and cultured with phytohemagglutinin Concanavalin A and interleukin-27days,The mutant frequencies of TCR gene were detected by flow cytometry.Results TCR genes mutation increases with the dose incense in the cultured the rat lymphocytes 7 days and it exists favourable dose-response relationship.Data were found to be fitted out optimal by a quadratic polynomial dose-response modle,which could be described by regression equation： TCRMF= TCRMF=1.615＋9.979D＋1.712D2（F=146.781,P0.01,R2adj=0.867）.Conclusion The favourable dose-response relationship between TCRMF induced by X ray and dose can be found from this study.