应用TPPA和TRUST试验检测梅毒螺旋体抗体及其临床意义

Detection of Treponema pallidun Antibody by Using TPPA and TRUST Tests and Analysis of Their Clinical Significance

目的比较梅毒螺旋体明胶颗粒凝集试验(TPPA)与甲苯胺红不加热血清试验(TRUST)两种梅毒血清学试验的检测结果 ,以及两者联合检测的临床意义。方法对我院2009年1-12月1003例门诊及住院患者血清标本进行TPPA和TRUST试验检测梅毒特异性抗体,并比对分析检测结果。结果 1003份患者血清标本中TPPA法检出梅毒抗体血清学阳性420例(41.87%);TRUST法检出梅毒抗体血清学阳性226例(22.53%),两方法比较,差异有统计学意义(P<0.01)。结论 TPPA法的特异性优于TRUST法,可用作为梅毒的确证试验,TRUST滴度分析可作为疗效观察,判定复发或再感染指征。两方法结合检测对加强梅毒监测,有效控制梅毒流行有着积极的临床意义。

Objective To compare the results of the two kinds of serodiagnosis in detection of Treponema pallidun infection,and to explore the clinical significance of Treponema pallidum particle agglutination （TPPA） assay combined with toluidine red unheated serum test （TRUST）.Methods Treponema pallidun antibody was detected in 1,003 serum samples collected from outpatients and inpatients of The First People’s Hospital of Chenzhou from January to December,2009 by TPPA assay and TRUST,and the detection results of the two methods were compared and analyzed.Results There were statistically significant differences in positive rate between TPPA assay （41.87%） and TRUST test （22.53%）,（P〈0.01）.Conclusions The specificity of TPPA assay is superior to that of TRUST test,which could be considered as the confirmatory test on Treponema pallidun infection.TRUST could be considered as a detection indicator of relapse and re-infection of Treponema pallidun.Combination of the two tests in diagnosis of Treponema pallidun infection could improve the monitoring of Treponema pallidun and control the prevalence of Treponema pallidun.