多药耐药相关蛋白-3表达与肝癌耐药的相关性

Relationship between multi-drug resistance relative protein-3 and liver caner multi-drug resistance

目的探讨多药耐药相关蛋白-3(MRP-3)的表达与肝癌耐药的相关性。方法应用RT-PCR方法检测正常肝细胞L-02、肝癌细胞BEL及肝癌耐药细胞HepG2/ADM中MRP-3mRNA的差异表达,再将MRP-3反义及正义寡核苷酸片段分别用脂质体转染进肝癌耐药细胞HepG2/ADM中,用MTT法、流式细胞仪及RT-PCR检测转染后细胞内阿霉素(ADM)的荧光强度、耐药指数及MRP-3mRNA的表达情况。结果肝癌耐药细胞HepG2/ADM中MRP-3mRNA的表达明显高于正常肝细胞L-02和肝癌细胞BEL(P<0.05)。MRP-3反义转染进耐药细胞后,可明显降低细胞内ADM的耐药指数,提高细胞内ADM浓度(P<0.05),细胞内MRP-3mRNA的表达较未转染细胞下降了62.5%(P<0.05)。结论 MRP-3的高表达可能是导致肝癌多药耐药的机制之一。

Objective To explore the relationship between the expression of multi-drug resistance relative protein-3 （MRP-3） and liver cancer multi-drug resistance.Methods The expression of MRP-3 in normal hepatocyte L-02,hepatocarcinoma cell BEL and hepatocarcinoma multi-drug resistance cell line HepG2/ADM were detected by RT-PCR.Anti-and oligonucleotide of MRP-3 were transfected by lipidosome into HepG2/ADM,intra-cellular doxorubicin （ADM） fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were detected by MTT,flow cytometry,and RT-PCR.Results The expression of MRP-3 in HepG2/ADM was higher than that of normal hepatocyte L-02 and hepatocarcinoma cell BEL.After transfected with anti-oligonucleotide MRP-3,ADM fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were all obviously decreased （P〈0.05）.Conclusions Higher expression of MRP-3 maybe one of the mechanisms of hepatocarcinoma multi-drug resistance.