抑癌基因PTEN随增龄在大鼠肾脏组织的表达

Alteration of the expression of PTEN in rats with aging

目的:研究与张力蛋白同源、第10号染色体缺失的磷酸酶基因(Phosphatase and tensin homologue deleted on chromosome 10,PTEN)随增龄在大鼠肾组织内表达的变化,并探讨其在肾脏衰老中的作用。方法:分别留取3、12、24月龄Wistar大鼠肾组织(n=6),石蜡切片CD34免疫组化染色观察肾组织微血管生成;western blot和免疫组化染色检测大鼠肾组织PTEN表达。结果:western blot显示,随增龄大鼠肾组织蛋白表达水平逐渐升高,24月龄大鼠表达水平显著高于3,12月龄大鼠(P<0.05);免疫组化显示PTEN在大鼠肾小球和肾小管均有表达,24月龄大鼠肾小球和肾小管阳性面积百分比均显著高于3、12月龄大鼠(P<0.05)。随增龄大鼠肾组织内出现微血管生成障碍,24月龄大鼠肾小球毛细血管和管周毛细血管密度均显著低于3、12月龄大鼠(P<0.05);相关分析显示,二者与大鼠肾组织PTEN蛋白表达水平均呈负相关(r=-0.761和-0.613,P<0.05)。结论:随增龄大鼠肾组织PTEN蛋白表达呈现上升趋势,可能通过影响血管生成参与肾脏衰老过程。

Objective：To investigate the alteration of the expression of PTENin rats with aging.Methods：Wistar rats were divided into 3 groups：3-month-old group,12-month-old group and 24-month-old group（n=6）.Immunohistochemistry of CD34 was employed to detecte angiogenesis in the kidneys with aging.The protein expression of PTEN was examined by Western Blot.Immunohistochemistry was also employed to detect the expression of PTEN in the kidneys with aging.Results：（1）Compared with 3-month-old rats and 12-month-old rats,the level of PTEN protein expression in kidneys of 24-month-old rats was higher than that of 3-month-old rats and 12month-old rats,as examined by Western Blot（P〈0.05）;（2） With aging,the positive percentages of PTEN expression in the kidneys were increased,as detected by Immunohistochemistry（P 〈0.05）;（3）With aging,impaired angiogenesis was found in renal tissues of Wistar rats.At the age of 24 months,the glomerular capillary density and the peritubular capillary density were lower than that of 3-month-old rats and 12-month-old rats（P〈0.05）,which were correlated negatively with the protein expression of PTEN in kidneys（r=-0.761 and-0.613,P〈0.05）.Conclusion：PTEN could promote age-related renal insenecence through affecting angiogenesis.