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相较正常结肠黏膜,Hath1在非粘液性结肠腺癌中的表达下调 被引量：4

Hath1 expression is down-regulated in colon non-mucinous adenocarcinomas compared with the expression in normal colon mucosa
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摘要 目的:研究Hath1基因在非粘液性结肠癌中的表达。方法:从48例非粘液性结肠腺癌患者的标本中取材,石蜡包埋切片,H&E染色,观察正常结肠黏膜、癌旁黏膜和结肠癌组织中杯状细胞数量的变化。用定量实时RT-PCR和Western blot的方法,检测这3种组织中Hath1蛋白和Muc2蛋白的表达。结果:在结肠癌组织中未发现杯状细胞;结肠癌组织中Hath1 mRNA、Hath1蛋白、Muc2 mRNA和Muc2蛋白的表达均显著下调。结论:Hath1基因的表达下调,可能与非粘液性结肠腺癌的发生有关。 Objective：To investigate the Hath1 expression in colon non-mucinous adenocarcinomas.Methods：Sections of paraffinembedded tissues were used to investigate the goblet cell population of normal colon mucosa,mucosa adjacent colon cancer and colon cancer samples which came from 48 patients with colon non-mucinous adenocarcinoma.Hath1 and Muc2 expression in these samples were tested by immunohistochemistry,quantitative real-time reverse transcription-PCR and Western blot.Results：There were not any goblet cell to be found in colon cancr.The levels of Hath1 mRNA and Hath1,Muc2 mRNA and Muc2 were significantly down-regulated in colon cancer.Conclusion：The down-regulation of Hath1 expression may correlate with colon carcinogenesis.
作者 朱代华 龚建平 任柯 孙建明 魏思东
机构地区 重庆医科大学附属第二医院普通外科 重庆医科大学附属第二医院肝胆外科
出处 《重庆医科大学学报》 CAS CSCD 北大核心 2010年第5期666-669,共4页 Journal of Chongqing Medical University
基金 重庆市自然科学基金(编号:CSTC 2007BB5262)
关键词 结肠癌 Hath1基因 病因学 Colon carcinoma Hath1 gene Etiology
分类号 R730.2 [医药卫生—肿瘤]
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参考文献15

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同被引文献75

  • 1倪坤,黄红彦.Atoh1基因在耳聋康复中的研究进展[J].中国康复,2006,21(6):410-412. 被引量：4
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重庆医科大学学报
2010年 第5期

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