摘要
目的探讨Neurogenesin-1(Ng1)基因对脊髓损伤后功能恢复的影响。方法将36只大鼠随机分为实验组和对照组,每组18只。采用改良Allen法制备大鼠胸10脊髓损伤模型后,通过Alzct微泵分别向实验组和对照组持续转染入Ng1重组质粒和空白质粒。术后各时间点BBB评分系统监测大鼠运动功能恢复情况,并于术后第2周和第4周时分别取材,应用神经细胞特异性免疫荧光双标染色和组织学观察Ng1基因对内源性神经干细胞分化的影响以及脊髓组织病理变化情况。结果自损伤后1周起实验组大鼠的BBB评分明显高于对照组。组织学观察实验组脊髓形态恢复较好逐渐趋于正常。实验组脊髓组织中新分化的神经元细胞数较对照组明显增多,同时新分化的星型胶质细胞数显著减少。结论Ng1基因能够诱导脊髓损伤后内源性神经干细胞分化为神经元,促进脊髓运动功能的修复。
Objective -To explore the effects of Neurogenesin-1 (Ng1) gene on functional recovery meter spinal cord injury (SCI). Methods Thirty-six rats were randomly divided into two groups, experimental group and control group, with 18 each. T10 spinal cord contusion injury was created in all these rats using modified Allen's method. Ngl recombinant plasmld and blank plasmid were transfected into the damaged areas respectively by Alzet pumps. At different time points after SCI, BBB score was obtained to monitor the recovery of motor function. At 14 or 28 days after the injury, nerve cell specific double immunofluorescent labeling and histopathologic examinations were performed to study the differentiation of adult endogenous neural precursors and the pathological changes after SCI, Results From 1 to 4 weeks after SCI, BBB scores in the Ngl group were significantly higher than those in the control group. Histologic observation showed that spinal cord morphology in the Ngl Group was better than that in the control Group and was close to normal spinal cord tissue. The mean numbers of BrdU +/MAP-2 + newborn neurons were significantly higher than that in the control group, while the numbers of BrdU +/GFAP + newborn astrocytes decreased in the Ngl group. Conclusion Ngl gene could induce endogenous neural stem cells to differentiate into neurons and hence promote motor function recovery after spinal cord injury.
出处
《中华手外科杂志》
CSCD
北大核心
2010年第3期185-189,共5页
Chinese Journal of Hand Surgery
基金
基金项目:国家自然科学基金资助项目(30772201)