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一株H5N2亚型鹦鹉源禽流感病毒分子进化分析 被引量：1

Molecular Analysis of an Avian Influenza Virus Isolate of H5N2 Subtype from Parrot
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摘要 2005年在广东进行流行病学调查时分离到一株鹦鹉源禽流感病毒,经鉴定为H5N2亚型禽流感病毒(A/Parrot/Guangdong/268/2005)。该毒株的HA裂解位点附近的氨基酸序列为RETRGLF,只含有一个碱性氨基酸,符合低致病性禽流感病毒的HA裂解位点附近氨基酸序列的分子特征;与H5N2亚型禽流感代表毒株相比,该毒株HA和NA基因的糖基化位点、HA基因的受体结合位点编码区、NA基因的耐药性位点均未发生变异。将该毒株全基因组序列与GenBank已公布的19株H5N2亚型禽流感病毒株的相应序列进行比较分析并绘制系统进化树后发现:其与低致病性禽流感毒株A/Pheasant/NJ/1355/1998(H5N2)-like的亲缘关系最近,位于以A/Chicken/Pennsylvania/1/1983(H5N2)为代表的美洲进化分支。 In 2005,an avian influenza virus stain was isolated from Parrot in Guangdong,which was then genotyped as H5N2 subtype and designated as A/Parrot/Guangdong/268/2005. According to the current OIE definition on the low-pathogenicity of avian influenza virus,the strain was recognized as a low pathogenic avian influenza virus due to the presence of one basic amino acid residue at the HA cleavage site. Some molecular characteristics of the virus,such as potential glycosylation sites in HA and NA,receptor binding sites of HA,and drug resistance site of NA,showed no variations. To analyze molecular evolution of this strain,we selected the sequences of H5N2 subtype AIVs from GenBank and established the phylogenetic trees. Our results indicated that this strain shared the highest homologies with the H5N2 LPAI isolate A/Pheasant/NJ/1355/1998-like. Phylogenic analysis revealed the isolate,together with A/Chicken/Pennsylvania/1/1983（H5N2）,belonged to America lineages and clustered with A/Pheasant/NJ/1355/1998-like.
作者 单芬 韦良孟 韦艳梅 徐成刚 罗开健 任涛 辛朝安 焦培荣 廖明
机构地区 华南农业大学兽医学院农业部动物疫病防控重点开放实验室广东省动物源性人兽共患病预防与控制重点实验室
出处 《病毒学报》 CAS CSCD 北大核心 2010年第3期183-188,共6页 Chinese Journal of Virology
基金 教育部创新团队项目(IRT0723) 国家肉鸡产业技术体系项目(nycytx-42-G3-03) 广东省自然科学基金项目(8451064201001132) 广东省科技项目(2007A020300011)
关键词 禽流感 H5N2亚型 鹦鹉 分子进化分析 avian influenza H5N2 subtype parrot molecular evolution analysis
分类号 S852.65 [农业科学—基础兽医学]
  • 相关文献

参考文献14

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共引文献14

同被引文献12

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二级引证文献6

病毒学报
2010年 第3期

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