二乙烯亚胺对脊髓灰质炎病毒Ⅱ型Sabin株的灭活效果

Inactivation Effect of Binary Ethylenimine on Type Ⅱ Poliovirus Sabin Strain

目的观察二乙烯亚胺(Binary ethylenimine,BEI)对脊髓灰质炎病毒Ⅱ型Sabin株的灭活效果。方法在不同温度(37、33、28和25℃)下采用4种浓度(0.002、0.001、0.0005和0.00025mol/L)的BEI及0.0925mg/ml的甲醛对脊髓灰质炎病毒Ⅱ型Sabin株进行灭活,采用微量滴定法测定病毒感染性滴度,ELISA法检测灭活病毒液D抗原含量,并进行病毒灭活验证试验。结果在37℃条件下,0.002mol/L的BEI能在48h内完全灭活病毒,灭活后的病毒液D抗原含量回收率为90.6%,显著高于甲醛灭活的回收率(60.6%)。经验证,灭活彻底,无活病毒存在。结论 BEI对脊髓灰质炎病毒Ⅱ型Sabin株的D抗原破坏小,能较好地保持疫苗的有效成分。

Objective To observe the inactivation effect of binary ethylenimine（BEI）on type Ⅱ poliovirus Sabin strain.Methods Sabin strain was inactivated at various temperatures（37,33,28 and 25℃）with BEI at various concentrations（0.002,0.001,0.0005 and 0.00025 mol /L）,and determined for infectious titer of virus by microtitration and for D antigen contents by ELISA,using those inactivated with formaldehyde as control,based on which the verification test on virus activation was performed.Results Sabin strain was completely inactivated with 0.002 mol /L BEI at 37℃ for 48 h.The recovery rate of D antigen content of the inactivated virus was 90.6%,which was significantly higher than that inactivated with formaldehyde（60.6%）.No live virus was detected by verification test.Conclusion BEI showed less damage to D antigen of type Ⅱ poliovirus Sabin strain and good protection to active constituents of vaccine.