糖尿病大鼠肾脏肾素及其受体表达改变

Renal expression of renin and renin receptor in the streptozotocin-induced rats

目的研究链脲佐菌素糖尿病大鼠肾脏肾素及肾素受体表达变化情况。方法 12只雄性Wistar大鼠随机分为对照组(N组)、糖尿病组(DM组)。大鼠左下腹腔注射链脲佐菌素(STZ)60mg/kg建立糖尿病模型。测定第4、8周尿蛋白。8周后心脏取血检测血糖、血肌酐、血钠、血钾;取肾脏行PAS染色观察病理改变;免疫组化检测肾脏肾素和肾素受体表达水平;RT-PCR检测肾脏肾素和肾素受体mRNA表达水平。结果 DM组4周始相同时间段尿蛋白较N组升高(P<0.01),8周时DM组24h尿蛋白较N组明显升高(P<0.01);DM组8周时肾小球损伤指数明显高于N组(P<0.05);免疫组化和RT-PCR检测显示DM组肾素表达较N组明显升高(P<0.05),而肾素受体表达差异无统计学意义(P>0.05)。结论肾脏肾素表达增加可能参与糖尿病肾脏早期损伤。

Objective To investigate the expression of renin and renin receptor in the kidneys of streptozotocin-induced rats.Methods Twelve male Wistar rats were randomly assigned to normal control group（n=6,group N）and diabetic group（n=6,group DM）.Streptozotocin（60 mg/kg）was administrated intraperitoneally to induce diabetes.Urine creatinine and proteinuria were measured at week 4,8.At week 8 animals were sacrificed and blood glucose,serum creatinine （SCr）,serum sodium and serum potassium were assayed.Renal pathological changes were quantified with glomerular injury index on PAS-stained sections.Protein and mRNA levels of renin and renin receptor expression were analyzed by immunohistochemical staining and RT-PCR respectively.Results The proteinuria and glomerular injury index in diabetic rats were significantly increased when compared to those in normal rats at week 8.Protein and mRNA expression of renin expression in kidneys of diabetic rats was up-regulated,however,renin receptor expression was comparable among two groups.Conclusion Elevated expression of renal renin maybe involve in the progression of diabetic renal damage.