RNAi对肝癌细胞放射增敏的实验研究

Experimental research of RNAi to the enhanced radiotherapy sensitivity of liver cancer cell

目的探讨ATM基因表达沉默对肝癌细胞辐射增敏的影响。方法采用含ATM干扰片段的慢病毒感染HepG2细胞,将细胞制成单细胞悬液,给予不同剂量照射,照射后继续培养细胞,研究RNAi前后HepG2细胞照射后继续细胞集落形成率,利用Graphpad prism5.0软件拟和线性二次曲线模型和单击多靶模型,得出放射生物学参数。结果 HepG2细胞干扰前后D0值分别为:3.37±0.03、3.50±0.06,Dq值分别为1.73±0.02、1.21±0.03,α/β值分别0.72±0.11、3.52±0.30,D0、Dq、α/β值差异均有统计学意义。放射增敏比SER=1.37。结论 RNAiATM基因可以改变肝癌细胞的放射生物学参数,达到放射增敏的目的。ATM有望成为肝癌患者治疗的新靶点。

Objective To explore the influence of the silent express of ATM gene on the enhanced sensitiveity of radiotherapy to the liver cancer cell.Methods Different doses of radiotherapy were given to the single cell suspensions of different concentration made of viras infected HepG2 cells with ATM interference.Cells were cultivated after radiotherapy,To investigated HepG2 cells colong forming efficiency after radiotherapy before and after RNAi.Radiation biological parameter were obtained after the linear quadratic curve model and click multifarget model were astallished.Results The values of D0,Dq and α/β with HepG2 cells before and after RNAi were all significance.The ratio of enhanced radiotherapy sensitivity was 1.37.Conclusion The goal of enhanced radiotherapy sensitivity can be achieved by changing the radiation biological parameter of liver cancer cell through RNAi of ATM gene,ATM will hopefully be the new target of treatment in the liver cancer patients.