脂联素基因+45位核苷酸T/G多态性与冠心病的相关性研究

Study on the relationship between adiponectin +45 nucleotide T/G polymorphism and coronary heart disease

目的探讨脂联素基因+45位核苷酸T/G多态性与冠心病的相关性。方法对365例研究对象进行选择性地冠状动脉造影,根据造影结果和临床资料分为3组:冠心病(CHD)组221例,冠状动脉粥样硬化(CAS)组44例和正常对照(NC)组100例;用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法检测脂联素基因+45位核苷酸T/G多态性的基因型。结果 (1)所有研究对象中,脂联素基因+45位核苷酸TT基因型占46.85%,TG基因型占42.47%,GG基因型占10.68%。T等位基因频率为68.08%,G等位基因频率为31.92%。(2)CHD组TT、TG、GG基因型和T、G等位基因频率与非CHD组及正常对照组比较差异无统计学意义(P>0.05)。结论脂联素基因+45位核苷酸T/G多态性与冠心病无明显相关,提示脂联素基因可能不是冠心病的易感基因。

Objective To investigate the genotype distribution of adiponectin ＋45 nucleotide T/G polymorphism and the relationship between the polymorphism and coronary heart disease. Methods 365 subjects were included：221 coronary heart disease patients （CHD group）,44 coronary artery artherosclerosis patients （CAS group） and 100 healthy subjects （normal control,NC group）.All subjects were confirmed by coronary angiography,and were genotyped for the polymorphism of adiponectin by polymerase chain reaction-restrictive fragment length polymorphism （PCR-RFLP） assay. Results （1）The TT,TG and GG genotype frequencies of adiponectin ＋45 nucleotide T/G polymorphism among all subjects were 46.85%,42.47% and 10.68%,respectively.The allelic frequencies were 68.08% for T allele and 31.92% for G allele.（2）The frequencies of TT,TG and GG genotype between CHD group and non-CHD group or NC group had no significant difference（P0.05）.The frequencies of T and G allele had no significant difference,too. Conclusion There was no relationship of the gene polymorphism of adiponectin ＋45 nucleotide T/G with coronary heart disease,and adiponectin gene may not be a susceptibility gene to coronary heart disease.