乳腺癌中MTA1、MMP-9表达与临床病理研究

Clinic pathological study of metastasis-associated protein1 (MTA1),Matrix Metalloproteinase-9(MMP-9) expression in breast cancer

目的探讨MTA1、MMP-9表达量与乳腺癌淋巴转移的关系及临床意义,初步探讨MTA1、MMP-9之间的关系。方法采用免疫组化法对45例浸润性乳腺导管癌中MTA1、MMP-9的表达情况进行检测。结果对实验结果进行定量分析,采用χ2检验,Fisher精确检验。乳腺癌中MTA1、MMP-9阳性表达率为71%、64%。MTA1与淋巴结转移相关,与年龄、肿瘤大小、ER、PR、c-erbB-2无相关性;MTA1蛋白定位变化可能与乳腺癌淋巴结转移相关(P<0.05);MMP-9与淋巴结转移成正相关(P<0.05),与PR呈负相关(P<0.05),与年龄、肿瘤大小、ER、c-erbB-2无相关性;MTA1与MMP-9有正相关趋势(P=0.057),但无统计学意义;MTA1(+)/MMP-9(+)表达与乳腺癌淋巴转移显著性相关(P<0.05)。结论 MTA1高表达促进乳腺癌细胞的淋巴结转移,MTA1蛋白定位变化与乳腺癌淋巴结转移相关;MMP-9的高表达促进乳腺癌淋巴结转移;MMP-9与孕激素受体表达呈负相关;发生淋巴结转移时乳腺癌组织中的MMP-9升高主要由间质细胞产生;MTA1、MMP-9两者联合表达是乳腺癌高度浸润的生物学标志。

Objective To investigate the relationship between the expression of MTA1,MMP-9 protein and lymph node metastasis in breast cancer.The relationships between the expressions of MTA1,MMP-9 protein and the clinical parameters of breast carcinoma were analyzed.To initiatory explore the relationship between MTA1,MMP-9 protein.Methods SP immunohistochemical technique was used to detect the expressions of MTAI and MMP-9 protein among the 45 human breast cancer samples.Results The positive rates of MTA1,MMP-9 protein were 71.0%,64.0% respectively.Analysis of MTA1 over-expression indicated there hadn′t relationships between age,tumor size,ER,PR and c-erb-B2 and MTA1.But MTA1 was significantly correlated with lymphatic metastasis（P0.05）.MMP-9 over-expression was significantly correlated with lymphatic metastasis（P0.05） and was inversely correlated with PR（P0.05）.There had not relationships between MMP-9 and age,tumor size,ER and c-erb-B2.The co-expression of MTAI and MMP-9 was significantly correlated with lymphatic metastasis（P0.05）.Conclusion The over-expression of MTA1 stimulates the lymph node metastasis of breast cancer and the orientation variety of MTA1 protein is related to the metastasis of breast cancer.The over-expression of MMP-9 stimulates the metastasis of lymph node of breast cancer and a negative correlation was found between MMP-9 and PR.The co-expression of MTAI and MMP-9 was the biological character of breast cancer high aggressiveness.