反相高效液相色谱法同时测定不同产地仙茅中仙茅苷和仙茅苷乙的含量

Simultanous determination of curculigoside and curculigoside B in the rhizome of Curculigo orchioides from different regions by HPLC-DAD

目的测定不同产地仙茅中仙茅苷和仙茅苷乙的含量,以期为仙茅药材生产和临床应用提供科学的依据。方法根据仙茅在我国的地理分布特点,采集12个不同地区产仙茅药材,并收集了6个商品药材,应用HPLC-DAD技术测定仙茅苷和仙茅苷乙的含量。结果不同产地的仙茅药材、采集药材和商品药材间仙茅苷和仙茅苷乙的含量存在较大差异。12个采集样品中,仙茅苷的含量范围为0.028%~0.538%,仙茅苷乙的含量范围为0.103%~0.330%;6个仙茅商品药材中,仙茅苷的范围为0.161%~0.733%,仙茅苷乙的含量为0.033%~0.123%,商品药材中仙茅苷相对含量明显高于采集样品。结论不同地区产仙茅药材中仙茅苷和仙茅苷乙的含量不同,表明仙茅药材的品质可能与地理位置、气候、采收时间、生长年限和加工、贮藏等因素有关。

Objective To determine the content of curculigoside and curculigoside B in the rhizome of Curculigo orchioides from different regions,and provide scientific basis for production and clinical application of these medicinal materials.Methods According to the characteristic of geographical distribution of Curculigo orchioides,12 medicinal materials from different locations as well as 6 commercial medicinal materials were collected.The method of HPLC-DAD was used to determine the content of curculigoside and curculigoside B.Results There have significant varieties in the content of curculigoside and curculigoside B among 12 collected Curculigo orchioides samples and 6 commercial medicinal materials.For collecting samples,the content of curculigoside and curculigoside B were 0.028%~0.538% and 0.103%~0.330% respectively.For commercial samples,the content of curculigoside and curculigoside B were 0.161%~0.733% and 0.033%~0.123%,respectively.The contents of curculigoside were higher in commercial samples than in collecting samples.Conclusion There have significant varieties in the content of curculigoside and curculigoside B in 12 collected Curculigo orchioides samples which indicated that the quality of Curculigo orchioides may be related with geographic location where it grows,climates,growing and collecting times,and the method of process and storage.