HPLC测定宣肺清热合剂中盐酸麻黄碱与盐酸伪麻黄碱含量

Determination of ephedrine and pseudoephedrine in Xuanfei Qingre mixture by HPLC

目的建立宣肺清热合剂（麻黄、苦杏仁、浙贝母、黄芩等）中有效成分盐酸麻黄碱和盐酸伪麻黄碱的含量测定方法。方法采用高效液相色谱法,色谱柱Agilent ZORBA×SB-C18（5μm,4.6 mm x 250 mm）,流动相为乙腈-0.02 mol/L磷酸二氢钾（用磷酸调pH至2.7）,（4：100,含0.1%三乙胺）,流速：1.2 ml/min,检测波长：207 nm。结果盐酸麻黄碱在11.04~222.8μg/mL（r=0.999 9）,盐酸伪麻黄碱在8.27~165.4μg/ml（r=0.999 9）的浓度范围内均线性关系良好,回收率均在95%~105%之间,RSD均小于3%。结论本方法操作简便、重现性好,可以更有效地控制制剂的质量。

Objective To set up a method of simultaneously determining the contents of ephedrine and pseudoephedrine in Xuanfei Qingre mixture（Herba Ephedrae,Semen Armeniacae amarum,Bulbus Fritillaria thunbergii,Radix scutellaria,etc）.Methods The HPLC-quantitative analysis was carried out on a column of Agilent ZORBA× SB-C18 by using a mobile phase of acetonitrile-0.02 mol/L potassium dihydrogen phosphate（adjusted with phosphoric acid pH=2.7）,（4100,with 0.1% triethylamine）under a flow rate of 1.2 ml/min.207 nm was selected as the wavelength of detector.Results The linear range of ephedrine was 11.04～222.8 μg/ml（r=0.999 9）and pseudoephedrine＇s was 8.27～165.4 μg/ml（r=0.999 9）.The recoveries of them were all between 95% and 105%（RSD3%）.Conclusion This method is simple,reproducible and specific.