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气相色谱法检查依托咪酯注射液中二甘醇的含量

Determination of Diethylene glycol in Etomidate Injection by GC
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摘要 目的建立依托咪酯注射液中二甘醇检查方法。方法采用气相色谱法,以丙三醇为内标,色谱柱为INNOWAX毛细管柱(30m×0.53mm×1μm),汽化室温度220℃,程序升温:初始温度为120℃,保持3分钟;以每分钟10℃的速度升至230℃,保持5min。载气:氮气,4mL·min^-1;检测室温度250℃,尾吹30mL·min^-1,进样量1μL。结果上述测定方法能使内标(丙三醇)、1,2-丙二醇、1,3-丙二醇和二甘醇良好分离,在11μg·mL^-1-167μg·mL^-1的范围内浓度(C)与峰面积响应值(f=A/A内)有良好的线性关系,r=0.9995;进样精密度、日内测定精密度、日间测定精密度的RSD%分别为1.1%、0.9%和1.0%;平均回收率(n=9)为100.9%,RSD为1.5%;方法定量限和检测限分别为0.56μg·mL^-1,0.17μg·mL^-1。结论气相色谱法可以用于检查控制依托咪酯注射液中二甘醇的含量。 OBJECTIVE To determine Diethylene glycol in Etomidate Injection by GC.METHODS GC was applied to quantitative analysis.Chromatographic conditions were: INNOWAX column(30m×0.53mm×1μm);Flame ionization detector(FID);Column temperature: Maintaining the temperature of column at 120℃ for 3 minutes,then raising the temperature at a rate of 10℃ per miniutes to 230℃ for 5 minutes;Injector temperature: 220℃;Detector temperature: 240℃;Nitrogen was used as carrier gas,the flow rate was 4 mL·min^-1.An internal standard was glycerol.RESULTS The calibration curve was linear in the range of 11μg·mL^-1~167μg·mL^-1.The average recoveries(n=9) was 100.9%.CONCLUSION The established method is accuracy,sensitive and simple.
出处 《海峡药学》 2010年第5期81-82,共2页 Strait Pharmaceutical Journal
关键词 二甘醇 气相色谱法 Diethylene glycol GC
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参考文献3

  • 1O'Brien KL,Selanikio JD,HecdiverC.Epidemic of pediatric deaths from acute renal failure caused by diethylene glycol poisoning[J].JAMA,1998,279 (15):1175.
  • 2Eder Anne F,McGrath Cindy M,Dowdy Yonne G.Ethylene glycol poisoning:toxicokineti c and analytical factors affecting laboratory diagnosis[J].Clin Chem,198,44:168.
  • 3O'Neil MJ,Smith Ann,Heckhnan PE,et al.The Merck Index:An Encyclopedia of Chemical,Drugs,and Biologicals.13th ed.New Jersey:Merck Res Lab Division of Merck&Co.,Inc.2001.549.

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