鸡血清对人恶性肿瘤细胞增殖能力的影响

The effect of chicken serum on the proliferation of human tumor cells

目的:了解鸡血清对人恶性肿瘤细胞增殖能力的影响。方法:常规细胞培养后,以10%小牛血清为对照,以不同浓度的鸡血清为检测样品,采用四甲基偶氮唑蓝(MTT)法、丽丝胺罗丹明B(SRB)法及台盼蓝拒染活细胞计数法测定肿瘤细胞的增殖情况并相互比较;光学显微镜下观察肿瘤细胞形态变化,进行组织病理学评价。结果:MTT及SRB法结果显示肿瘤细胞增殖,而活细胞计数法结果(鸡血清抑制肿瘤细胞的增殖)与之相悖,组织病理学观察显示不同浓度的鸡血清对部分肿瘤细胞造成损伤并有细胞碎片形成,同活细胞计数法结果一致。结论:鸡血清作为一种异源血清对人肿瘤细胞具有杀伤作用,而MTT法和SRB法无法检测到这种杀伤作用。

Objective： To comprehend the effect of chicken serum on the reproductive activity of tumor cells. Methods： After the routine cell culture, using the 10% calf serum as the control group, the different concentration of chicken serum as the detection sample, the generation of tumor cells was determined and compared with each other by means of MTT, SRB and trypan blue viable count method ; the changes of the tumor cells＇ appearance were observed and evaluated with optical microscope. Results： The tumor cells had proliferated by both MTT and SRB method. However the viable count method （the chicken serum inhibites its proliferration） showed opposite. And the histopathology suggests the different of chicken serum not only could lead to injuery on tumor cells but also the formation of cell debris, the same as the retults of viable count method. Conclusion：As a heterogenous serum,the chicken serum could wound the human tumor cells, but the action can＇t be tested by MTT or SRB method.