乙肝后肝硬化患者血清CTGF、PDGF、TGF-β_1测定的意义

The Clinical Significance of Determination of Serum CTGF,PDGF and TGF-β_1 Levels in Patients with Post-Hepatitis B Liver Cirrhosis

目的:探讨乙型肝炎后肝硬化患者血清CTGF、PDGF、TGF-β1水平的变化及临床意义。方法:将50例乙肝后肝硬化患者按不同的病情分为轻度、中度及重度三组;设体检健康人45名作为对照组。血清TGF-β1水平采用放射免疫分析;CTGF及PDGF则采用ELISA测定。并将测定结果进行统计分析。结果:结果显示,血清CTGF水平轻度组与对照组比较略有升高,但无统计学意义(P>0.05);中度组患者该指标水平则显著高于对照组(P<0.05);重度组水平更为显著(P<0.01)。且发现其递增规律与肝硬化病情的严重程度呈明显的平行关系。PDGF测定值显示,轻度组水平显著高于对照组(P<0.05),中度和重度两组水平则较对照组升高更为显著(P均<0.01)。其水平的递增关系也与病情的严重程度相一致。TGF-β1水平的变化趋势同PDGF。结论:本文患者三项血清指标水平的变化与乙肝后肝硬化的发病及病情进展有关;其测定有助于了解本病的发生机制和预后评估。

Objective To explore the clinical significance of changes of serum connective tissue growthfactor（CTGF）,Platelet-derived growth factor（PDGF）and transforming growth factor-β1（TGF-β1）levels in patients with post-hepatitis B liver cirrhosis.Methods Serum TGF-β1（with RIA）,CTGF and PDGF（with ELISA）levels were determined in 50 patients with liver cirrhosis（mild,n=15 moderate n=16 severe,n=19）and 45 controls.Results The serum level of CTGF in patients with mild post-hepatitis B liver cirrhosis were in significantly higher than those in the controls（P0.05）.The serum level of CTGF were significantly higher in patients with moderate（P0.05）and severe（P0.01）post-hepatitis B liver cirrhosis than those in the cortrols.Serum levels of PDGF were significant higher in patients with mild post-hepatitis B liver cirrhosis than those in the controls（P0.05）and even higher in patients with moderate and severe post-hepatitis B liver cirrhosis（P0.01）.The serum level of TGF-β1 were also significantly higher in all the patients than those in the controls（P0.05,P0.01,P0.01）.Conclusion The changes of those 3 markers serum levels were related to the progress of post-hepatitis B liver cirrhosis and the determination was helpful for outcome prediction.