拟南芥盐胁迫响应启动子的生物信息学分析

BIOINFORMATICAL ANALYSIS OF SALINITY-RESPONSIVE PROMOTERS IN ARABIDOPSIS THALIANA

本研究使用生物信息学方法分析了拟南芥盐胁迫响应的顺式作用元件。首先分析盐胁迫0.5 h、1 h的拟南芥全基因组芯片,得到627个盐胁迫上调基因和282个下调基因。然后使用MEME软件分析了盐胁迫响应基因的启动子,得到10个保守元件。通过显著性分布分析表明Motif_1\Motif_8\Motif_10显著分布于上调基因启动子中;Motif_1\Motif_10显著不分布于盐胁迫下调基因启动子中。Motif_1元件是G-box元件(ABRE(ABAResponsive Element)元件),大量分布于盐胁迫上调基因启动子中,广泛参与盐胁迫过程中ABA依赖的信号转导途径。Motif_8、Motif_10分别类似于ABRE元件和DRE(Dehydration-Responsive Element)元件,但由于和ABRE、DRE元件的保守序列不尽相同,Motif_8\Motif_10很有可能是新的盐胁迫响应元件。本研究对于研究拟南芥在盐胁迫应答过程中在转录水平上发生的调控过程具有重要帮助作用.

Salinity-responsive motifs were analyzed with multiple bioinformatic softwares in this study.627 salinity up-regulated genes and 282 down-regulated genes were identified from the microarray data about 0.5h and 1h high salinity stress in Arabidopsis.The upstream 1000bp promoter regions of all salinity-regulated genes retrieved from TAIR were used for finding conserved motifs by MEME software and 10 putative such motifs were identified.Motif_1 was indentified as a G-box element,which is one of the ABA-responsive elements that functions in ABA-dependent gene expression under abiotic stress,and overrepresents in salinity up-regulated promoters.Motif_8 and Motif_10 were also overrepresented in salinity up-regulated promoters,and the conserved sequences of these two motifs were similar to ABRE and DRE,respectively.Since conserved sequences of ABRE and DRE are not present in Motif_8 and Motif_10,the two motifs might be new salinity-responsive elements.This study will help studying the regulation process in transcriptional level during the responses to salt stress in Arabidopsis.