玫瑰海棠组培快繁技术的研究

STUDY ON TECHNOLOGY OF TISSUE CULTURE AND RAPID-PROPAGATION ON BEGONIA ELATIOR HYBRIDS

以玫瑰海棠(Begonia elatior hybrids)叶片为外植体,探讨影响玫瑰海棠分化的主要因素,正交实验结果表明:影响玫瑰海棠分化的主要因素是培养基中的植物生长素和分裂素的浓度比值,诱导玫瑰海棠叶片不定芽的培养基为MS+6-BA1.8 mg.L-1+NAA0.1 mg.L-1+糖30 g.L-1+琼脂6.5 g.L-1,诱导不定芽增殖培养基为MS+6-BA1.2mg.L-1+NAA0.1 mg.L-1+糖30 g.L-1+琼脂6.5 g.L-1,生根培养基是1/2MS+IAA0.1 mg.L-1+糖30 g.L-1+琼脂6.5 g.L-1,蔗糖对玫瑰海棠的分化影响效果不明显。

Using lamina of the Begonia elatior hybrids as the explant,this paper probes the key factors that affect lamina of begonia elatior hybrids polarize,the results tested by orthogonal design shows that key factors affecting begonia elatior hybrids polarize is the rate of phytohormone and cytokinins,the appropriate medium for adventitious buds of lamina is MS＋6-BA1.8 mg·L-1＋NAA 0.1 mg·L-1 ＋sugar 30 g·L-1 ＋agar 6.5 g·L-1,the medium of multiplication is MS＋6-BA 1.2 mg·L-1＋NAA 0.1 mg·L-1 ＋sugar 30 g·L-1＋agar 6.5 g·L-1,the rootage medium is 1/2MS＋IAA 0.1 mg·L-1 ＋sugar 30 g·L-1＋agar 6.5 g·L-1,the effect of sugar affecting the Begonia elatior hybrids polarize would be no patency.