糖尿病大鼠TNF-α表达与大血管病变的关系

Relationship between the Expression of TNF-α and the Macro-Vascular Disease in Diabetic Rats

目的:通过观察糖尿病大鼠不同时间段颈动脉中肿瘤坏死因子(TNF-α)表达变化趋势及颈动脉内中膜厚度(CIMT),探讨TNF-α与糖尿病的大血管病变之间的关系。方法:将大鼠分为正常对照组(A组n=18)、糖尿病组(B组n=18),分别于实验的4周、8周、12周,采用酶联免疫吸附法、RT-PCR法测定血清及颈动脉中TNFα-水平,同时测定CIMT。结果:与A组比较,B组大鼠随TNF-α表达增强CIMT显著增加(P<0.05),大鼠颈动脉TNF-αmRNA的表达与CIMT存在明显正相关关系。结论:TNF-α参与了糖尿病大血管病变的发生发展。

Objective： To explore the relationship of TNF-α and the macro-vascular disease in diabetes by observing the changing trend of TNF-α expression in the carotid artery at different time points in diabetic rats and the carotid intima-media thickness（CIMT）.Methods： The rats were randomly divided into a normal control group（group A n=18） and a diabetes group（group B n=18） to determine the serum and the TNF-α in the carotid artery by enzyme-linked immunosorbentassay（ELISA） and RT-PCR,and meanwhile to determine the carotid intima-media thickness（CIMT）at the 4th,8th and 12th weeks of the experiment.Results： Compared with A,the carotid intima-media thickness（CIMT）of the rats in group B increased significantly with the TNF-α expression increasing（P〈0.05）.The expression of TNF-α mRNA was positive correlation with CIMT.Conclusion：TNF-α may participate in the onset and development of macro-vascular disease in diabetes.