摘要
目的:通过观察雌激素对老年大鼠脂肪干细胞(ADSCs)体外增殖和成骨分化能力的影响,进一步明确雌激素水平和增龄变化与骨代谢相关性。方法:选取18月龄雌性SD大鼠ADSCs,雌激素刺激浓度为10-7mol/L,采用MTT法,茜素红染色及生化检测雌激素对ADSCs体外增殖与成骨分化能力的影响。结果:成骨诱导21d镜下观测含雌激素组茜素红染色阳性面积大于常规诱导组,非诱导组染色阴性。RT-PCR半定量检测显示含雌激素组较常规诱导组OCN表达强烈。结论:雌激素能增强老年大鼠ADSCs体外增殖与诱导成骨分化能力。
Objective:To observe and reveal the effects of 17-β estradiol on modulating in vitro osteogenic differentiation and proliferation of adipose tissue-derived mesenchymal stem cells(ADSCs)isolated from 18-month-old female Sprague Dawleg rats.Methods:17-β estradiol was investigated for modulating in vitro osteogenic differentiation of ADSCs of the third generation isolated from a 18-month-old female rat.The capacity of 17-β estradiol on cell osteogenic differentiation was observed by alizarin red staining,RT-PCR and detection of morphological and molecular biology.Results:17-β estradiol could effectively enhance ADSCs' proliferation.After ADSCs' exposure to osteogenic differentiation medium supplemented with 17-β estradiol,the expression of osteogenic markers was significantly improved.Conclusion:17-β estradiol can modulate the differentiation and proliferation,and potentially improve the efficiency of ADSCs in stem cell-based tissue engineering and regeneration.However,further study is needed to verify the regulatory functions of 17-β estradiol on ADSCs' differentiation.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2010年第3期340-342,共3页
Journal of Oral Science Research