NF-κB、TGF-β1在糖尿病大鼠心肌及主动脉中的表达

The Expression of NF-κB and TGF-β1 in the Myocardium and Aorta of STZ-induced Diabetic Rats

目的:探讨链脲佐菌素(STZ)诱导的糖尿病(DM)大鼠心肌及主动脉核转录因子-κB(NF-κB)、转化生长因子-β1(TGF-β1)的表达。方法:用HE及MASSON染色观察DM大鼠心肌及主动脉的病理改变,用免疫组化的方法检测上述免疫因子的表达。结果:DM大鼠心肌、主动脉NF-κB、TGF-β1表达均明显高于正常对照组(CON),差异有统计学意义(P<0.01)。结论:NF-κB和TGF-β1在DM心血管并发症的发生发展机制中起重要作用。

Objective To observe the expression of Nuclear factor-κB（NF-κB）and Transforming growth factor-β1（TGF-β1）in the myocardium and aorta of diabetic rats induced by STZ.Methods 20 male Wister rats were divided into two groups.10 rats without any treatment were served as control group.10 rats were diabetic rats induced by STZ.At 34 weeks,20 male Wister rats were sacrificed for pathological and Immunohistochemical study.The pathological changes were assessed by HE and Masson staining.The expression of cytokines（NF-κB and TGF-β1）in the myocardium and aorta of diabetic rats was assessed with immunohistochemistry.Results In comparison with control group,the expressions of NF-κB and TGF-β1 in the myocardium and aorta of diabetic rats induced by STZ were significantly increased（both P0.01）.Conclusions The NF-κB and TGF-β1 may play a critical role in the pathogenesis of the chronic diabetic cardiovascular complications.