厌氧复合菌群JZ-1降解拟除虫菊酯的特性和组成多样性研究

Composite Diversity and Degradation Characteristics of Synthetic Pyrethroids(SPs) Degradation Anaerobic Microbial Community JZ-1

从农药厂废水中分离到能够降解多种拟除虫菊酯的复合菌群JZ-1,对该菌群降解特性研究结果表明,最佳降解条件为pH7、温度30℃;在最佳条件下培养15d,对100mg·L-1甲氰菊酯、氟氯氰菊酯、氯氰菊酯的降解率分别为53.27%、33.36%、41.39%。对该菌群的16S rDNA进行扩增和RFLP及测序分析结果表明,该菌群含有丰富的细菌资源,该菌群的优势种群包括红假单胞菌属(Rhodopseudomonas)和紫单孢菌科细菌(Porphyromonadaceae bacterium)。该研究为拟除虫菊酯的降解菌资源的挖掘和利用提供了理论参考。

Synthetic pyrethroids（SPs）degrading microbial community JZ-1 was domesticated by selective culture.Degrading experiments showed the optimum degrading conditions were pH 7,30 ℃.JZ-1 degraded fenpaprothren,cyfluthrin and cypermethrin with a concentration of 100 mg·L-1 up to 53.27%,33.36%,41.39% in 15 days in optimum conditions,respectively.The nearly complete 16S rDNA were amplified from JZ-1 using bacterial universal primers 27F and 1492R.The restriction analysis was performed following separate digestion with enzyme Rsa I,Which showed there were six clusters in JZ-1,Which meaned there were plenty of degradation bacterial resources in JZ-1.The representative 16S DNA sequences of six clusters were sequenced,and showed that the major clusters were Rhodopseudomonas and Porphyromonadaceae bacterium,respectively.This study contributes to screen and apply SPs degrading organisms for SPs biodegradation.