RAPD鉴定棉花抗(耐)黄萎病品种(系)的遗传变异研究

Detection of Genetic Variation of Upland Cotton Cultivars (Lines)Resistant(Tolerant)to Verticillium dahliae with RAPD Technique

利用ＲＡＰＤ分子标记技术，结合已知系谱信息，对国内外不同来源的２５个抗（感）黄萎病的棉花品种（系）进行特征及特性分析。从被测试的４０个引物中选取对２５个棉花品种（系）ＤＮＡ扩增表现多态性的２６个随机引物，构建２５个棉花品种（系）ＤＮＡ指纹图谱，并进一步分析了遗传多样性。结果表明：供试的２５个棉花品种（系）可划分为４个类群，这与其系谱来源及抗黄萎病的抗源来源基本吻合。第Ⅰ类为国外引入的抗黄萎病品种；第Ⅱ类为陕棉、辽棉系统；第Ⅲ类为遗传基础复杂，从病圃定向选择培育的抗黄萎病品种（系）；第Ⅳ类为长江流域感黄萎病品种“太仓１２１”。该研究从ＤＮＡ水平上揭示了中国现有的抗（耐）黄萎病品种（系）的遗传真实性。

Genetic variation of 25 cotton cultivars (lines)resistant or susceptible to Verticillium dahliae in upland cotton was detected with RAPD molecular marker technology.DNA fingerprintings of these 25 cultivars were constructed using 26 primer amplification products which were selected from 40 ones appeared polymorphism to DNA amplification of 25 cotton varities(lines) and their genetic diversity were analysed.25 cotton cultivars (lines)could be classified into four subgroups,group Ⅰ included introduced cultivars resistant to Verticillium dahliae ,group Ⅱ included cotton varieties in Shanxi and Liaoning Provinces,resistant varieties (lines) of group Ⅲ which were derived from disease nursey under directional seletion had complex genetic basis,and the last group is Taicang 121,a susceptible one.The four groups classified by cluster analysis with RAPD are basically accorded with their pedigree origins and origins of resistant resources.The genetic relationship among resistant(tolerant)cultivars to Verticillium dahliae was established at DNA level with RAPD technique.